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HPLC Fingerprinting of Chloroform Extracts of Seven Ethnomedicinal Plants of Mizoram, India
Corresponding Author(s) : Longjam Shantabi
Asian Journal of Chemistry,
Vol. 33 No. 12 (2021): Vol 33 Issue 12, 2021
Abstract
The present work was done for seven ethnomedicinal plants used by the inhabitants of Mizoram in order to investigate the presence of various phytoconstituents. The root-stock of Alocasia indica, leaves of Bidens pillosa, Chromolaena odorata, Elaeagnus caudata and Spilanthes acmella, the latex of Carica papaya and rhizomes of Curcuma caesia were dried and powdered. The chloroform extract of each sample were prepared by soaking dried powdered samples in chloroform for 72 h. The extracts were filtered using Whatman filter paper No. 42 (125 mm). The filtrates of plant extracts were preserved at 4-5 ºC for further process. Crude extracts of selected plants parts were analyzed using TLC coupled to HPLC fingerprinting, which gives some prominent and moderate peaks with different retention time, which may be a bioactive compounds.
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- S. Tripathi, Glob. J. Arch. Anthropol., 10, 555796 (2019); https://doi.org/10.19080/GJAA.2019.10.555796
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- R. Raghavendra Rao, J. Ethnobotany, 8, 14 (1996).
- V. Duraipandiyan, M. Ayyanar and S. Ignacimuthu, BMC Compl. Altern. Med., 6, 35 (2006); https://doi.org/10.1186/1472-6882-6-35
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- WHO, General Guidelines for Methodologies on Research and Evaluation of Traditional Medicine, World Health Organization, Geneva, Switzerland (2000).
- P.K. Rai and H. Lalramnghinglova, Ethnobotan. Leaflets, 14, 274 (2010).
- J.B. Harborne, Phytochemical Methods-A Guide to Modern Techniques of Plant Analysis, Chapman and Hall Ltd.: London, pp. 1-129 (1998).
- J.H. Doughari, Eds.: Venketeshwer Rao, Phytochemicals: Extraction Methods, Basic Structures and Mode of Action as Potential Chemotherapeutic Agents, Phytochemicals-A Global Perspective of their Role in Nutrition and Health, InTech: Rijeka, Croatia (2012).
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- A.K. Nadkari, Indian Material Media, Popular Prakashan Pvt. Ltd.: Bombay (1976).
- S.K. Bhatt and V.R. Saxena, Indian Drugs, 17, 210 (1980).
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- P. Geissberger and U. Sequin, Acta Trop., 48, 251 (1991); https://doi.org/10.1016/0001-706X(91)90013-A
- V.F. Andrade-Neto, M.G.L. Brandao, F.Q. Oliveira, V.W.D. Casali, B. Njaine, M.G. Zalis, L.A. Oliveira and A.U. Krettli, Phytother. Res., 18, 634 (2004); https://doi.org/10.1002/ptr.1510
- V. Steenkamp and M.C. Gouws, S. Afr. J. Bot., 72, 630 (2006); https://doi.org/10.1016/j.sajb.2006.02.004
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References
S. Tripathi, Glob. J. Arch. Anthropol., 10, 555796 (2019); https://doi.org/10.19080/GJAA.2019.10.555796
S.K. Pareek, Eds.: P.L. Gautam et al., Medicinal Plants in India: Present Status and Future Prospects. Prospects of Medicinal Plants, Indian Society for Plant Genetic Resources, NBPGR campus: New Delhi, pp. 5-14 (1996).
S. Mukhopadhyay, Eds.: P.L. Gautam et al., Conservation, Protection and Biodiversity of Medicinal Plants, Indian Society for Plant Genetic Resources, NBPGR Campus: New Delhi, pp. 15-28 (1996).
R. Raghavendra Rao, J. Ethnobotany, 8, 14 (1996).
V. Duraipandiyan, M. Ayyanar and S. Ignacimuthu, BMC Compl. Altern. Med., 6, 35 (2006); https://doi.org/10.1186/1472-6882-6-35
L. Shantabi, G.C. Jagetia, M. Vabeiryureilai, K. Lalrinzuali, J. Biodivers. Biopros. Dev., 1, 136 (2014); https://doi.org/10.4172/2376-0214.1000136
X.H. Fan, Y.Y. Cheng, Z.L. Ye, R.C. Lin and Z.Z. Qian, Anal. Chim. Acta, 555, 217 (2006); https://doi.org/10.1016/j.aca.2005.09.037
C. Tistaert, B. Dejaegher and Y.V. Heyden, Anal. Chim. Acta, 690, 148 (2011); https://doi.org/10.1016/j.aca.2011.02.023
WHO, General Guidelines for Methodologies on Research and Evaluation of Traditional Medicine, World Health Organization, Geneva, Switzerland (2000).
P.K. Rai and H. Lalramnghinglova, Ethnobotan. Leaflets, 14, 274 (2010).
J.B. Harborne, Phytochemical Methods-A Guide to Modern Techniques of Plant Analysis, Chapman and Hall Ltd.: London, pp. 1-129 (1998).
J.H. Doughari, Eds.: Venketeshwer Rao, Phytochemicals: Extraction Methods, Basic Structures and Mode of Action as Potential Chemotherapeutic Agents, Phytochemicals-A Global Perspective of their Role in Nutrition and Health, InTech: Rijeka, Croatia (2012).
K.R. Kirtikar and B.D. Basu, Indian Medicinal Plants, M/s Bishen Singh Mahendrapal Singh: Dehradun, India, Edn. 2, vol. 4, pp. 2617 (1975).
A.K. Nadkari, Indian Material Media, Popular Prakashan Pvt. Ltd.: Bombay (1976).
S.K. Bhatt and V.R. Saxena, Indian Drugs, 17, 210 (1980).
M. Sawnliana, The Book of Mizoram Plants, Lois Bet, Chandmari, Aizawl, India, Edn. 1 (2003).
P. Geissberger and U. Sequin, Acta Trop., 48, 251 (1991); https://doi.org/10.1016/0001-706X(91)90013-A
V.F. Andrade-Neto, M.G.L. Brandao, F.Q. Oliveira, V.W.D. Casali, B. Njaine, M.G. Zalis, L.A. Oliveira and A.U. Krettli, Phytother. Res., 18, 634 (2004); https://doi.org/10.1002/ptr.1510
V. Steenkamp and M.C. Gouws, S. Afr. J. Bot., 72, 630 (2006); https://doi.org/10.1016/j.sajb.2006.02.004
M.M. Iwu, A.R. Duncan and C.O. Okunji, New Antimicrobials of Plant Origin, In: Perspectives on New Crops and New Uses, ASHS Press: Alexandria, USA, pp. 457-462 (1999).
C.P. Kala, J. Ethnobiol. Ethnomed., 1, 11 (2005); https://doi.org/10.1186/1746-4269-1-11
A. Chakraborty, R.K.B. Devi, S. Rita, Kh. Sharatchandra and T.I. Singh, Indian J. Pharmacol., 36, 148 (2004).