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Vol. 29 No. 3 (2017): Vol 29 Issue 3

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Determination of Yield of Enzymatic Hydrolysis of Vegetable Oils by Near-Infrared Spectroscopy

https://doi.org/10.14233/ajchem.2017.20257
G.M. Rodrigues
Departamento de Tecnologia, Universidade Estadual de Maringá (UEM), Av. Angelo Moreira da Fonseca 180, Umuarama, PR, 87506-370, Brazil
C. Silva
Departamento de Tecnologia, Universidade Estadual de Maringá (UEM), Av. Angelo Moreira da Fonseca 180, Umuarama, PR, 87506-370, Brazil

Corresponding Author(s) : C. Silva

camiladasilva.eq@gmail.com

Asian Journal of Chemistry, Vol. 29 No. 3 (2017): Vol 29 Issue 3

Abstract

In this work, a rapid and environmental friendly method to evaluate the yield of hydrolysis reactions of vegetable oils in relation to the production of free fatty acids is proposed, using near-infrared (NIR) spectroscopy and multivariate calibration. For calibration of the analytical method hydrolyzed samples of soybean oil with different free fatty acids contents were analyzed by partial least squares (PLS) regression. For the calibration and validation steps, values for root-mean-square error of 0.87 and 0.88, respectively were reported. The method was tested to predict the free fatty acids content in enzymatically hydrolyzed samples of different vegetable oils (soybean, macauba, sunflower and corn). It was observed from the results that the method presented is effective for predicting free fatty acids concentration in the range from 1.5 to 80.5 wt %, with prediction errors lower than 1.0 wt %. The correlation between the values observed for the reference methodology and those predicted by the NIR/PLS method presented coefficients of determination (R2) > 0.97 for the all oils studied, demonstrating the effectiveness of the method to assess the progress of hydrolysis of vegetable oils with different fatty acid profiles. The results obtained demonstrate that the proposed method can be employed for monitoring and rapid screening analysis of free fatty acid production by the hydrolysis of vegetable oils.

Keywords

Free fatty acids Hydrolysis Near-infrared Multivariate calibration
Rodrigues, G., & Silva, C. (2016). Determination of Yield of Enzymatic Hydrolysis of Vegetable Oils by Near-Infrared Spectroscopy. Asian Journal of Chemistry, 29(3), 571–575. https://doi.org/10.14233/ajchem.2017.20257
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