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Vol. 33 No. 9 (2021): Vol 33 Issue 9, 2021

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Comparative Studies of Phytochemicals Analysis and in vitro Antioxidant Properties of Various Solvent Extracts of Sphaeranthus indicus Collected from three Different Climatic Zones of Central India

https://doi.org/10.14233/ajchem.2021.23413
Pooja N. Akh
1Department of Bioscience and Biotechnology, Banasthali Vidyapith, Rajasthan-304022, India 2Department of Pharmacy, Barkatullah University, Bhopal-462026, India *Corresponding author: E-mail: anupampathak@yahoo.com
Veena Sharma1
1Department of Bioscience and Biotechnology, Banasthali Vidyapith, Rajasthan-304022, India 2Department of Pharmacy, Barkatullah University, Bhopal-462026, India *Corresponding author: E-mail: anupampathak@yahoo.com
Anupam K. Pathak2
1Department of Bioscience and Biotechnology, Banasthali Vidyapith, Rajasthan-304022, India 2Department of Pharmacy, Barkatullah University, Bhopal-462026, India *Corresponding author: E-mail: anupampathak@yahoo.com

Asian Journal of Chemistry, Vol. 33 No. 9 (2021): Vol 33 Issue 9, 2021

Abstract

In present study, the total phenolic, flavonoid, tannin contents and the antioxidant activity of various solvent extracts of Sphaeranthus indicus collected from three different regions of central India were assessed. Plants extracts were prepared using Soxhlation method, while the total phenols, flavonoids and tannins were measured by the spectrophotometric method. DPPH, metal chelating, nitric oxide, superoxide oxide scavenging activity and FRAP, reducing power, total antioxidant assays were also evaluated. The highest phenolic contents 268.22 GAE equivalent, flavonoids 441.33 QE equivalents and tannin content was 120.32 tannic acid equivalents (mg/g) obtained from ethanolic extracts of sample SIEE-1 compared to other two plants extract. Similarly, the highest flavonoid contents was observed in SIAE-1 aqueous extract and lowest in SIAE-2 and SIAE-3. Extract of SIEE-1 possessed maximum antioxidant potentiality and SIAE-2 shown the least antioxidant activity in all assays. It could be concluded that different agroclimatic conditions have effects on the total phenolics, flavonoids, tannin contents and antioxidant potentiality of S. indicus plant.

Keywords

Sphaeranthus indicus Antioxidant activity Total phenolic Total flavonoids Total tannin Climatic zone.
N. Akh, P., Sharma1, V., & K. Pathak2, A. (2021). Comparative Studies of Phytochemicals Analysis and in vitro Antioxidant Properties of Various Solvent Extracts of Sphaeranthus indicus Collected from three Different Climatic Zones of Central India. Asian Journal of Chemistry, 33(9), 2237–2243. https://doi.org/10.14233/ajchem.2021.23413
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References
  1. R.A. Roberts, D.L. Laskin, C.V. Smith, F.M. Robertson, E.M.G. Allen, J.A. Doorn and W. Slikker, Toxicol. Sci., 112, 4 (2009); https://doi.org/10.1093/toxsci/kfp179
  2. U.S. Ramjith, B. Roopitha and C.M. Jacob, Asian J. Pharm. Clin. Res., 6, 228 (2013).
  3. O.A. Adesanoye, A.E. Adekunle, O.B. Adewale, A.E. Mbagwu, A.A. Delima, S.A. Adefegha, O.R. Molehin and E.O. Farombi, Toxicol. Ind. Health, 32, 47 (2016); https://doi.org/10.1177/0748233713498436
  4. A. Adeyanju, O.R. Molehin, E. Ige, L. Adeleye and O. Omoniyi, J. Appl. Pharm. Sci., 8, 106 (2018); https://doi.org/10.7324/JAPS.2018.81014
  5. D. Krishnaiah, R. Sarbatly and R. Nithyanandam, Food Bioprod. Process., 89, 217 (2011); https://doi.org/10.1016/j.fbp.2010.04.008
  6. R.N. Chopra, S.L. Nayar and I.C. Chopra, Glossary of Indian Medicinal Plants, National Institute of Science Communication, New Delhi, Ed.1, p. 232 (1996).
  7. S.L. Bodhankar, S.D. Ambavade, N.A. Mhetre and V.D. Tate, Indian J. Pharm., 38, 254 (2006); https://doi.org/10.4103/0253-7613.27021
  8. S.K. Singh, K.M. Saroj, V.H. Tripathi, A.K. Singh and R.H. Singh, Int. J. Crude Drug Res., 26, 235 (1988); https://doi.org/10.3109/13880208809053925
  9. K.S. Dubey, A.H. Ansari and M. Hardaha, Asian J. Chem., 12, 577 (2000).
  10. A.R. Bafna and S.H. Mishra, ARS Pharmaceutica, 45, 281 (2004).
  11. S.C. Garg and H.L. Kasera, Fitoterapia, 54, 37 (1983).
  12. R.T. Mahajan, G.S. Chaudhari and M.Z. Chopda, Environ. Bulletin, 15, 61 (1999).
  13. F. Sadaf, R. Saleem, M. Ahmed, S.I. Ahmad and Navaid-ul-Zafar, J. Ethnopharmacol., 107, 161 (2006); https://doi.org/10.1016/j.jep.2006.02.022
  14. R.N. Yadava and S. Kumar, Fitoterapia, 70, 127 (1999); https://doi.org/10.1016/S0367-326X(98)00018-5
  15. P.P. Pujar, D.D. Sawaikar, S.R. Rojatkar and B.A. Nagasampagi, Fitoterapia, 71, 264 (2000); https://doi.org/10.1016/S0367-326X(99)00157-4
  16. S.R. Rojatkar and B.A. Nagasampagi, Phytochemistry, 31, 3270 (1992); https://doi.org/10.1016/0031-9422(92)83492-H
  17. V. Lodha, Indian Perfumer, 47, 29 (2003).
  18. M.S. Shekhani, P.M. Shah, K.M. Khan and Atta-ur-Rahman, J. Nat. Prod., 54, 882 (1991); https://doi.org/10.1021/np50075a025
  19. S.K. Singh, V.J. Tripathi and R.H. Sing, Indian Drugs, 26, 317 (1988).
  20. Ayurvedic Pharmacopoeia of India. Annony, Government of India, Ministry of Health and Family Welfare, Department of Indian System of Medicine and Homeopathy, Ed.: 1, vol. 3, Part 1, New Delhi, p. 21 (1998).
  21. V.L. Singleton and J.A. Rossi, Am. J. Enol. Vitic., 16, 144 (1965).
  22. T.J. Mabry, K.R. Markham and M.B. Thomas, The Systematic Identification of Flavonoids. Springer-Verlag: New York, p. 354 (1970).
  23. G.S. Kartashova and L.V. Sudos, Pharm. Chem. J., 31, 488 (1997); https://doi.org/10.1007/BF02464310
  24. A. Hagerman, M.I. Harvey and H.P.S. Makker, Quantification of Tannins in Tree Foliage-A Laboratory Manual, FAO/IAEA, Vienna, pp. 4-7 (2000).
  25. W. Brand Williams, M.E. Cuvelier and C. Berset, Lebensm. Wiss. Technol., 28, 25 (1995); https://doi.org/10.1016/S0023-6438(95)80008-5
  26. M.S. Blios, Nature, 181, 1199 (1958); https://doi.org/10.1038/1811199a0
  27. T.C.P. Dinis, V.M.C. Madeira and L.M. Almeida, Arch. Biochem. Biophys., 315, 161 (1994); https://doi.org/10.1006/abbi.1994.1485
  28. D.C. Garrat, The Quantitative Analysis of Drugs, Chapman & Hall Ltd., vol. 3, pp. 456-848 (1964).
  29. M. Nishikimi, N. Appaji Rao and K. Yagi, Biochem. Biophys. Res. Commun., 46, 849 (1972); https://doi.org/10.1016/S0006-291X(72)80218-3
  30. M. Oyaizu, Jpn. J. Nutr., 44, 307 (1986); https://doi.org/10.5264/eiyogakuzashi.44.307
  31. P. Prieto, M. Pineda and M. Aguilar, Anal. Biochem., 269, 337 (1999); https://doi.org/10.1006/abio.1999.4019
  32. I.F.F. Benzie and J.J. Strain, Anal. Biochem., 239, 70 (1996); https://doi.org/10.1006/abio.1996.0292
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