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Vol. 33 No. 11 (2021): Vol 33 Issue 11, 2021

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Graphene Oxide Nanoparticles Decorated Pencil Lead as Urea Sensing Electrode

https://doi.org/10.14233/ajchem.2021.23501
Saravjeet Singh
Department of Biomedical Engineering, Deenbandhu Chhotu Ram University of Science and Technology, Murthal-131039, India
Minakshi Sharma
Department of Zoology, Maharishi Dayanand University, Rohtak-124001, India
Geeta Singh
Department of Biomedical Engineering, Deenbandhu Chhotu Ram University of Science and Technology, Murthal-131039, India

Corresponding Author(s) : Geeta Singh

geetasingh.bme@dcrustm.org

Asian Journal of Chemistry, Vol. 33 No. 11 (2021): Vol 33 Issue 11, 2021

Abstract

In present study, a low-cost and steady electrochemical biosensor has been developed based on graphene oxide nanoparticles (GONPs), electrodeposited on the pencil lead electrode (PLE). The physical and morphological studies revealed the nano-scale range of GONPs that has an average grain size/layer thickness of 2.27 nm and agglomeration size of 90-120 nm. Urease enzyme was immobilized on PLE/GONPs electrode after surface treatment with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC)/N-hydroxysuccinimide (NHS) to impart stable peptide bond. Scanning electron microscopy (SEM) studies demonstrate the difference between unmodified and modified PLE electrodes and depict the spherical, circular and flaky-like morphology. The electrochemical analysis of urea considerably improved on the PLE/GONPs/Ur compared to the untailored PLE electrode. The reported urea biosensor operated in a linear dynamic range between 0.30-50 mM that attained a recognition limit of 0.06 mM and exhibited an advanced exposure sensitivity of 0.814 μA mM-1 cm-2.

Keywords

Urea Sensor Nanoparticles Pencil lead electrode Graphene oxide
Singh, S., Sharma, M., & Singh, G. (2021). Graphene Oxide Nanoparticles Decorated Pencil Lead as Urea Sensing Electrode. Asian Journal of Chemistry, 33(11), 2857–2863. https://doi.org/10.14233/ajchem.2021.23501
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