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Vol. 25 No. 9 (2013): Vol 25 Issue 9

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Influence of Different Organic Waste Materials on Hardening of Micropropagated Tea (Camellia sinensis L.) Clone 'Iran 100'

https://doi.org/10.14233/ajchem.2013.14196
Reza Azadi Gonbad
Department of Crop Science, Faculty of Agriculture, University Putra Malaysia, 43400 Serdang, Malaysia
Uma Rani Sinniah
Department of Crop Science, Faculty of Agriculture, University Putra Malaysia, 43400 Serdang, Malaysia
Maheran Abdul Aziz
Department of Agriculture Technology, Faculty of Agriculture, University Putra Malaysia, 43400 Serdang, Malaysia
Rosfarizan Mohamad
Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400 Serdang, Malaysia

Corresponding Author(s) : Uma Rani Sinniah

umarani@agri.upm.edu.my

Asian Journal of Chemistry, Vol. 25 No. 9 (2013): Vol 25 Issue 9

Abstract

In order to reduce mortalities of the tea plantlet Clone Iran 100 during the hardening stage after acclimatization from tissue culture, the effect of different organic materials on rooting of acclimatized plantlets was investigated. Organic materials used were urban waste material, farm yard manure, mushroom media and tea waste at the level of 0, 25, 50 or 75 % (v/v) per pot mixed with soil. Completely randomized block design with factorial arrangement was used and replicated thrice. During the acclimatization period, dry and fresh weight of the root and shoot, length of the root and shoot, number of leaves and roots along with survival rate were measured and recorded. The final outcome showed that tea waste treatment at the level of 25 % had the highest survival and is considered as the best treatment for hardening of tea.

Keywords

Acclimatization Media Survival Tea Tissue culture
Azadi Gonbad, R., Rani Sinniah, U., Abdul Aziz, M., & Mohamad, R. (2013). Influence of Different Organic Waste Materials on Hardening of Micropropagated Tea (Camellia sinensis L.) Clone ’Iran 100’. Asian Journal of Chemistry, 25(9), 4987–4990. https://doi.org/10.14233/ajchem.2013.14196
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