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Vol. 25 No. 9 (2013): Vol 25 Issue 9

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Investigation and Histopathological Evaluation of the Effects of Omeprazole on the Ischemia-Reperfusion Induced Oxidative Damage and DNA Mutation in Rat Ovarian Tissue

https://doi.org/10.14233/ajchem.2013.14150
Ismail Demiryilmaz
Department of General Surgery, Ibni Sina Hospital, Kayseri, Turkey
Nihal Cetin
Department of Pharmacology, Faculty of Medicine, Ataturk University, Erzurum, Turkey
Omer Erkan Yapca
Obstetrics and Gynecology Department, Sorgun State Hospital, Yozgat, Turkey
Durdu Altuner
Department of Pharmacology, Faculty of Medicine, Rize University, Rize, Turkey
Unal Isaoglu
Department of Obstetrics and Gynecology, Nene Hatun Obstetrics and Gynecology Hospital, Erzurum, Turkey
Fatma Betul Ozgeris
Department of Biochemistry, Faculty of Medicine, Ataturk University, Erzurum, Turkey
Muhammet Calik
Department of Pathology, Faculty of Medicine, Ataturk University, Erzurum, Turkey
Halis Suleyman
Department of Pharmacology, Faculty of Medicine, Ataturk University, Erzurum, Turkey

Corresponding Author(s) : Halis Suleyman

halis.suleyman@gmail.com

Asian Journal of Chemistry, Vol. 25 No. 9 (2013): Vol 25 Issue 9

Abstract

In this study, the biochemical and histopathological effects of omeprazole on ischemia-reperfusion (I/R) induced oxidative damage in rat ovarian tissue were investigated. The moment that the animals remained motionless in supine position was considered the appropriate time to perform surgery. The ovaries of the rats were reached through a 2.0-2.5 cm long vertical incision in the lower abdomen. Subsequently, in the omeprazole (OIR) and the control groups (I/RC), a vascular clip was placed in the lower part of the right ovary (the part where the ovary is attached to the uterus) and ischemia was maintained for 3 h. (No ischemia was applied in the healthy group.) After this period, the vascular clip was removed in order to provide reperfusion for 2 h. Afterwards, all the animals were terminated by high dose-anesthesia, the ovaries were removed and histopathological and biochemical studies were performed. Omeprazole has an antioxidant effect and it can have a protective function in the oxidative damage induced by ischemia-reperfusion. We have found that omeprazole prevents oxidative damage due to ischemia-reperfusion injury in rat ovarian tissue.

Keywords

Omeprazole Oxidative damage Ischemia-reperfusion Rat
Demiryilmaz, I., Cetin, N., Erkan Yapca, O., Altuner, D., Isaoglu, U., Betul Ozgeris, F., … Suleyman, H. (2013). Investigation and Histopathological Evaluation of the Effects of Omeprazole on the Ischemia-Reperfusion Induced Oxidative Damage and DNA Mutation in Rat Ovarian Tissue. Asian Journal of Chemistry, 25(9), 4943–4947. https://doi.org/10.14233/ajchem.2013.14150
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