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Vol. 25 No. 9 (2013): Vol 25 Issue 9

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Production of Transglycosylated Rutin Using Novel Cyclodextrin Glucanotransferase

https://doi.org/10.14233/ajchem.2013.14136
T. Sun
School of Perfume and Aroma Technology, Shanghai Institute of Technology, 100 Haiquan Road, Shanghai 201418, P.R. China
B. Jiang
State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, P.R. China
B.L. Pan
Chinese Institute of Food Science and Technology, Beijing 100006, P.R. China

Corresponding Author(s) : B.L. Pan

panbeilei@yahoo.com.cn; libsunt@yahoo.com

Asian Journal of Chemistry, Vol. 25 No. 9 (2013): Vol 25 Issue 9

Abstract

Besides physical and chemical methods, the enzymatic method could be applied on the modification of rutin molecule. In this study, the transglycosylation of rutin using novel cyclodextrin glucanotransferase (CGTase) produced by Bacillus sp. SK13.002 has been investigated. The optimization of enzymatic reaction conditions using orthogonal experimental design has also been carried out based on temperature, pH, the amount of enzyme cyclodextrin glucanotransferase and the reaction time. The results indicated that among these enzymatic reaction conditions reaction time is the most important variable. Under the optimized enzymatic reaction conditions of pH 5.5, temperature 35 ºC, 20 U cyclodextrin glucanotransferase per mL reaction system and 24 h of reaction time, the transglycosylation conversation rate of rutin could reach as high as 65.7 %. Furthermore the change of the components of transglycosylated rutins during the rutin transglycosylation has also been investigated.

Keywords

Rutin Transglycosylation Transglycosylated rutin Cyclodextrin glucanotransferase Orthogonal experimental design
Sun, T., Jiang, B., & Pan, B. (2013). Production of Transglycosylated Rutin Using Novel Cyclodextrin Glucanotransferase. Asian Journal of Chemistry, 25(9), 4889–4892. https://doi.org/10.14233/ajchem.2013.14136
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