Copyright (c) 2013 AJC
This work is licensed under a Creative Commons Attribution 4.0 International License.
Biosynthesis of Rubber in Cultured Cells of Sonchus oleraceus
Corresponding Author(s) : Norimasa Ohya
Asian Journal of Chemistry,
Vol. 25 No. 9 (2013): Vol 25 Issue 9
Abstract
Rubber (cis-1,4-polyisoprene) was detected in the leaves of Sonchus oleraceus by 1H-nuclear magnetic resonance spectroscopy. The rubber had a low molecular weight with a narrow molecular weight distribution centered around 1.0 × 105 Da. Callus cultures were initiated from the leaf parts of S. oleraceus and suspension cultures were established in Murashige and Skoog's medium with various concentrations of 1-naphthalene acetic acid (NAA) and benzylaminopurine (BA). The cell biomass was higher in the presence of 1-naphthalene acetic acid (0.1 mg/L) and benzylaminopurine (0.1 mg/L). The cultured cells contained trace amounts of rubber-like polymer identified by gel permeation chromatography.
Keywords
Download Citation
Endnote/Zotero/Mendeley (RIS)BibTeX
- L.G. Polhamus, Rubber, Botany, Production and Utilization, Leonard Hill Limited (1962).
- T.A. Coffelt, F.S. Nakayama, D.T. Ray, K. Cornish and C.M. McMahan, Ind. Crops Prod., 29, 326 (2009).
- K. Cornish, Hypoallergenic Natural Rubber Products from Parthenium argentatum (gray) and other Non-Hevea Brasiliensis Species, U.S. Patent 5717050 (1998).
- Campos-Lopez, E. Neavez-Camacho, M.A. Ponce-Velez and J.L. Angulo-Sanchez, Chemtech, 9, 50 (1979).
- R.A. Buchanan, I.M. Cull, F.H. Otey and C.R. Russell, Econ. Bot., 32, 146 (1978).
- T. Murashige and F. Skoog, Physiol. Plant., 15, 473 (1962).
- A. Subramaniam, Rubber Chem. Technol., 45, 346 (1972).
- Y. Tanaka, S. Kawahara, A.H. Eng, K. Shiba and N. Ohya, Phytochemistry, 39, 779 (1995).
- J. Tangpakdee, Y. Tanaka, W. Wititsuwannakul and N. Chareonthiphakorn, Phytochemistry, 42, 353 (1996).
References
L.G. Polhamus, Rubber, Botany, Production and Utilization, Leonard Hill Limited (1962).
T.A. Coffelt, F.S. Nakayama, D.T. Ray, K. Cornish and C.M. McMahan, Ind. Crops Prod., 29, 326 (2009).
K. Cornish, Hypoallergenic Natural Rubber Products from Parthenium argentatum (gray) and other Non-Hevea Brasiliensis Species, U.S. Patent 5717050 (1998).
Campos-Lopez, E. Neavez-Camacho, M.A. Ponce-Velez and J.L. Angulo-Sanchez, Chemtech, 9, 50 (1979).
R.A. Buchanan, I.M. Cull, F.H. Otey and C.R. Russell, Econ. Bot., 32, 146 (1978).
T. Murashige and F. Skoog, Physiol. Plant., 15, 473 (1962).
A. Subramaniam, Rubber Chem. Technol., 45, 346 (1972).
Y. Tanaka, S. Kawahara, A.H. Eng, K. Shiba and N. Ohya, Phytochemistry, 39, 779 (1995).
J. Tangpakdee, Y. Tanaka, W. Wititsuwannakul and N. Chareonthiphakorn, Phytochemistry, 42, 353 (1996).