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Vol. 25 No. 8 (2013): Vol 25 Issue 8

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A Novel Biosensor Based on Acetylecholinesterase/Chitosan-Graphene Oxide Modified Electrode for Detection of Carbaryl Pesticides

https://doi.org/10.14233/ajchem.2013.14019
Pengcheng Li
College of Chemistry and Chemical Engineering, Jiangxi Normal University, 99 Ziyang Road, Nanchang 330022, P.R. China
Yonghai Song
College of Chemistry and Chemical Engineering, Jiangxi Normal University, 99 Ziyang Road, Nanchang 330022, P.R. China
Souhui Chen
College of Chemistry and Chemical Engineering, Jiangxi Normal University, 99 Ziyang Road, Nanchang 330022, P.R. China
Mei Zhang
College of Chemistry and Chemical Engineering, Jiangxi Normal University, 99 Ziyang Road, Nanchang 330022, P.R. China
Li Wang
College of Chemistry and Chemical Engineering, Jiangxi Normal University, 99 Ziyang Road, Nanchang 330022, P.R. China

Corresponding Author(s) : Li Wang

lwang@jxnu.edu.cn; lwangsy2003@hotmail.com

Asian Journal of Chemistry, Vol. 25 No. 8 (2013): Vol 25 Issue 8

Abstract

A sensitive, fast and cheap sensor for quantitative determination of carbaryl pesticide using amperometric acetylcholinesterase biosensor based on graphene oxide-chitosan hybrid film was reported. This biosensor was fabricated by firstly depositing chitosan-graphene oxide hybrid film on glassy carbon electrode and subsequently assembling acetylcholinesterase on chitosan-graphene oxide hybrid film. The chitosan-graphene oxide hybrid film exhibited a good biocompatibility with acetylcholinesterase and effective immobilization of acetylcholinesterase. The resulted biosensor showed a good electrocatalytic activity toward oxidation of thiocholine, which was a product from the hydrolysis of acetylthiocholine catalyzed by acetylcholinesterase. The inhibition of carbaryl on the activity of acetylcholinesterase was proportional to carbaryl concentration in the range from 0.005 to 0.4 μM and from 1.0 to 5.0 μM, respectively. The detection limit was calculated to be about 4.0 nM. The biosensor provided a new promising tool for pesticide analysis.

Keywords

Acetylcholinesterase Graphene Chitosan Carbaryl Biosensor
Li, P., Song, Y., Chen, S., Zhang, M., & Wang, L. (2013). A Novel Biosensor Based on Acetylecholinesterase/Chitosan-Graphene Oxide Modified Electrode for Detection of Carbaryl Pesticides. Asian Journal of Chemistry, 25(8), 4444–4448. https://doi.org/10.14233/ajchem.2013.14019
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References
  1. D.W. Miwa, G.R.P. Malpass, S.A.S. Machado and A.J. Motheo, Water Res., 40, 3281(2006).
  2. F. Arduini, F. Ricci, C.S. Tuta, D. Moscone, A. Amine and G. Palleschi, Anal. Chim. Acta, 580, 155 (2006).
  3. A. Vakurov, C.E. Simpson, C.L. Daly, T.D. Gibson and P.A. Millner, Biosens. Bioelectron., 20, 2324 (2005).
  4. D. Du, S.Z. Chen, J. Cai and A.D. Zhang, Talanta, 74, 766 (2008).
  5. N. Sattarahmady, H. Heli and A.A. Moosavi-Movahedi, Biosens. Bioelectron., 25, 2329 (2010).
  6. S.Q. Liu, L. Yuan, X.L.Yue, Z.Z. Zheng and Z.Y. Tang, Adv. Power Technol., 19, 419 (2008).
  7. C. Chouteau, S. Dzyadevych, C. Durrieu and J.M. Chovelon, Biosens. Bioelectron., 21, 273 (2005).
  8. D. Du, W.J. Chen, W.Y. Zhang, D.L. Liu, H.B. Li and Y.H. Lin, Biosens. Bioelectron., 25, 1370 (2010).
  9. Z. Zheng, Y. Zhou, X. Li, S. Liu and Z. Tang, Biosens. Bioelectron., 26, 3081 (2011).
  10. A.P. Periasamy, Y. Umasankar and S.M. Chen, Sensors, 9, 4034 (2009).
  11. Y. Song, M. Zhang, L. Wang, L. Wan, X. Xiao, S. Ye and J. Wang, Electrochim. Acta, 56, 7267 (2011).
  12. D. Du, X. Huang, J. Cai and A.D. Zhang, Biosens. Bioelectron, 23, 285 (2007).
  13. G.D. Liu and Y.H. Lin, Anal. Chem., 78, 835 (2006).
  14. D. Du, X.X. Ye, J. Cai, J. Liu and A.D. Zhang, Biosens. Bioelectron, 25, 2503 (2010).
  15. L. Liu, S. Ryu, M.R. Tomasik, E. Stolyarova, N. Jung, M.S. Hybertsen, M.L. Steigerwald, L.E. Brus and G.W. Flynn, Nano Lett., 8, 1965 (2008).
  16. I. Calizo, A.A. Balandin, W. Bao, F. Miao and C.N. Lau, Nano Lett., 7, 2645 (2007).
  17. A.K. Geim and K.S. Novoselov, Nature, 446, 183 (2007).
  18. J.C. Meyer, A.K. Geim, M.I. Katsnelson, K.S. Novoselov, T.J. Booth and S. Roth, Nature, 446, 60 (2007).
  19. M. Ishigami, J.H. Chen, W.G. Cullen, M.S. Fuhrer and E.D. Williams, Nano Lett., 7, 1643 (2007).
  20. Y. Fang, S. Guo, C. Zhu, Y. Zhai and E. Wang, Langmuir, 26, 11277 (2010).
  21. C. Shan, H. Yang, D. Han, Q. Zhang, A. Ivaska and L. Niu, Biosens. Bioelectron, 25, 1070 (2010).
  22. M. Zhou, Y. Zhai and S. Dong, Anal. Chem., 81, 5603 (2009).
  23. C. Shan, H. Yang, J. Song, D. Han, A. Ivaska and L. Niu, Anal. Chem., 81, 2378 (2009).
  24. R.S. Dey and C.R. Raj, J. Phys. Chem. C, 114, 21427 (2010).
  25. W. Hong, H. Bai, Y. Xu, Z. Yao, Z. Gu and G. Shi, J. Phys. Chem. C, 114, 1822 (2010).
  26. L. Cao, Y. Liu, B. Zhang and L. Lu, ACS Appl. Mater. Interf., 2, 2339 (2010).
  27. J.M. Gong, T. Liu, D.D. Song, X.B. Zhang and L.Z. Zhang, Electrochem. Commun., 11, 1873 (2009).
  28. D. Du, S.Z. Chen, D.D. Song, H.B. Li and X. Chen, Biosens. Bioelectron, 24, 475 (2008).
  29. X. Kang, J. Wang, H. Wu, I.A. Aksay, J. Liu and Y. Lin, Biosens. Bioelectron., 25, 901 (2009).
  30. H. Xu, H. Dai and G. Chen, Talanta, 81, 334 (2010).
  31. W.S. Hummers Jr. and R.E. Offeman, J. Am. Chem. Soc., 80, 1339 (1958).
  32. F. Ricci, F. Arduini,A. Amine, D. Moscone and G. Palleschi, J. Electroanal. Chem., 563, 229 (2004).
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