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This work is licensed under a Creative Commons Attribution 4.0 International License.
Analysis of Chemical Constituents of Traditional Chinese Medicinal Formula Huang-Qi-Chi-Feng-Tang by High Performance Liquid Chromatography-Electrospray Ionization-Mass Spectrometry
Corresponding Author(s) : Bin Liu
Asian Journal of Chemistry,
Vol. 25 No. 8 (2013): Vol 25 Issue 8
Abstract
Huang-Qi-Chi-Feng-Tang, a three-herb traditional Chinese medicinal formula, has efficient effects on treatment of skin ulcer and pains. However, the active components of this compound Chinese prescription have not been fully explored. In this study, high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (HPLC-ESI-MS/MS) was applied for the qualitative analysis of major chemical constituents in Huang-Qi-Chi-Feng-Tang. Samples were separated on a Waters Symmetry Shield C18 column (150 mm × 4.6 mm) using a gradient system of 20 % methyl alcohol (A)-methyl alcohol (B). The precursor and product ions of analytes were monitored on a hybrid quadrupole linear ion trap mass spectrometer in negative mode. A total of 11 compounds including 3 chromones, 2 monoterpenoids, 5 isoflavone, 1 isoflavane were successfully identified or tentatively characterized based on retention time and MS spectra compared with those of standards or literature data. The results may contribute to the new drug development and illuminate the effective substance basis.
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References
H. Ma, Q. Zhu, X.Y. Sun and X.D. Li, Shi Zhen J. Trad. Chin. Med. Res., 9, 324 (1998).
Z.G. Li, Jilin J. Trad. Chin. Med., 26, 33 (2006).
L.H. Zhou and C.Z. Liu, Jiangxi J. Trad. Chin. Med., 35, 39 (2004).
H.X. Liu and C.P. Yin, Chin. J. Integr. Med. Cardio-/Cerebrovascular Dis., 6, 27 (2008).
C.A. Yuan, Hunan J. Trad. Chin. Med., 10, 18 (1994).
H.J. Zhang, P. Shen and Y.Y. Cheng, J. Pharm. Biomed. Anal., 34, 705 (2004).
Zh. X. Yan, X.H. Yang, J.B. Wu, H. Su, CH. Chen and Y. Chen, Anal. Chim. Acta, 691, 110 (2011).
J. Han, M. Ye, H. Guo, M. Yang, B.R. Wang and D.A. Guo, J. Pharm. Biomed. Anal., 44, 430 (2007).
S.J. Xu, L. Yang, M. Zhang and Z.T. Wang, Acta Pharm. Sin., 41, 852 (2006).
X. Zhang, H.B. Xiao, X.Y. Xue, Y.G. Sun and X.M. Liang, J. Sep. Sci., 30, 2059 (2007).
Q. Ma, J.L. Zhang, Y.X. Zhou, H.M. Lei and C.H. Wang, Chin. J. Anal. Chem., 34, 247 (2006).
X.G. He, L.Z. Lin and L.Z. Lian, J. Chromatogr. A, 75, 127 (1996).
L.W. Qi, Q.T. Yu, P. Li, S.L. Li, Y.X. Wang, L.H. Sheng and L. Yi, J. Chromatogr. A, 1134, 162 (2006).
L.W. Qi, P. Li, S.L. Li, L.H. Sheng, R.Y. Li, Y. Song and H.J. Li, J. Sep. Sci., 29, 2211 (2006).
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