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Macroporous Resin Column Simulated Moving Bed Chromatography Adsorption for Separation of Albiflorin and Paeoniflorin
Corresponding Author(s) : Wei Zhang
Asian Journal of Chemistry,
Vol. 25 No. 8 (2013): Vol 25 Issue 8
Abstract
D101 macroporous resin column simulated moving bed separation process of albiflorin and paeoniflorin isomers was applicated in the paper. Determine the simulated moving bed separation conditions of albiflorin and paeoniflorin, separation column for the D101 macroporous resin column (ID. 26 mm × 400 mm), simulated moving bed chromatographic operation mode :1-4-3, sample concentration: 250 mg/mL, sampling flow rate: = 2 mL/min, elution rate: = 20 mL/min, extraction flow rate: = 35 mL/min, switching time: = 25 min, elution and extract:methanol: water (v/v) = 20 : 80. Calculated according to the standard curve, mass concentration of paeoniflorin in product was 90 %, the yield was 78 %; albiflorin was 91 %, the yield was 75 %. Under these conditions, the system can be stable operation. The relevant factors that impact of simulated moving bed separation was also discussed in the paper.
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- X.-Y. Zhang, J.-H. Wang and X. Li, J. Shenyang Pharm. Univ., 18, 30 (2001).
- S.-F. Lu and Y.-J. Shi, Planta Med., 63, 323 (1997).
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- M. Kaneda, Y. Iitaka and S. Shibata, Tetrahedron, 28, 4309 (1972).
- T. Okubo, F. Nagai, T. Seto, K. Satoh, K. Ushiyama and I. Kano, Chem. Pharm. Bull., 23, 199 (2000).
- A. Sugaya, T. Suzuki, E. Sugaya, N. Yuyama, K. Yasuda and T. Tsuda, J. Ethnopharm., 33, 159 (1991).
- Q. Wang, R.X. Liu, H.Z. Guo, M. Ye, C.H. Huo, K.S. Bi and D. Guo, Chromatographia, 62, 581 (2005).
- B.-C. Lin, Fine Chem., 22, 110 (2005).
- W. Zhang and B.-C. Lin, Fine Chem., 24, 554 (2007).
- G. Paredes, M. Mazzotti, J. Stadler, S. Makart and M. Morbidelli, Adsorption, 7, 841 (2005).
- Y.-H. Liu, Y. Chen and J.-H. Yi, West China J. Pharmaceut. Sci., 17, 295 (2002).
- L. Zhou, Food Sci. Technol., 33, 152 (2008).
- X.-Y. Nie, C. Shang, Y.-L. Wang, B. Zhang and H.-B. Qu, Chinese Trad. Patent Med., 27, 350 (2005).
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- Y.-P. Xing, B.-C. Lin, W.-H. Zhou, X.-D. Tang and H. Gao, Fine Chem., 26, 666 (2009)
References
X.-Y. Zhang, J.-H. Wang and X. Li, J. Shenyang Pharm. Univ., 18, 30 (2001).
S.-F. Lu and Y.-J. Shi, Planta Med., 63, 323 (1997).
R. Sun, L.-L. Lv, S.-D. Guo and G.-Q. Liu, et al., J. Harbin Univ. Comm., 21, 406 (2005).
Z.-D. Ge, A.-W. Zhou and B. Wang, Chinese Pharmacol. Bull., 11, 303 (1995).
X.-S. Yan, H.-M. Li, Y.-Q. Peng and A.-P. Ming, Chinese J. Integrat. Trad. Western Med. Liver Diseases, 16, 18 (2006).
M. Kaneda, Y. Iitaka and S. Shibata, Tetrahedron, 28, 4309 (1972).
T. Okubo, F. Nagai, T. Seto, K. Satoh, K. Ushiyama and I. Kano, Chem. Pharm. Bull., 23, 199 (2000).
A. Sugaya, T. Suzuki, E. Sugaya, N. Yuyama, K. Yasuda and T. Tsuda, J. Ethnopharm., 33, 159 (1991).
Q. Wang, R.X. Liu, H.Z. Guo, M. Ye, C.H. Huo, K.S. Bi and D. Guo, Chromatographia, 62, 581 (2005).
B.-C. Lin, Fine Chem., 22, 110 (2005).
W. Zhang and B.-C. Lin, Fine Chem., 24, 554 (2007).
G. Paredes, M. Mazzotti, J. Stadler, S. Makart and M. Morbidelli, Adsorption, 7, 841 (2005).
Y.-H. Liu, Y. Chen and J.-H. Yi, West China J. Pharmaceut. Sci., 17, 295 (2002).
L. Zhou, Food Sci. Technol., 33, 152 (2008).
X.-Y. Nie, C. Shang, Y.-L. Wang, B. Zhang and H.-B. Qu, Chinese Trad. Patent Med., 27, 350 (2005).
H. Liu, M. Wan, Y. Hao, X.-Y. Zhu, X.-R. Ta and D.-Q. Zhang, J. Tianjin Univ. Trad. Chinese Med., 28, 142 (2005).
Y.-P. Xing, B.-C. Lin, W.-H. Zhou, X.-D. Tang and H. Gao, Fine Chem., 26, 666 (2009)