HPTLC Method Development and Validation of Secondary Metabolite-Thymol from Sufof-E-Mohazzil: A Unani Formulation

Avinash G. Patil; Aqsa Y. Ansari; Swapneel P. Koli; Darshana A. Patil

doi:10.14233/ajchem.2013.13806

Issue

Vol. 25 No. 7 (2013): Vol 25 Issue 7

Issue Published : January 18, 2013

This work is licensed under a Creative Commons Attribution 4.0 International License.

HPTLC Method Development and Validation of Secondary Metabolite-Thymol from Sufof-E-Mohazzil: A Unani Formulation

https://doi.org/10.14233/ajchem.2013.13806

Avinash G. Patil

Department of Botany-Herbal Sciences, Birla College, Kalyan-421 304, India

Aqsa Y. Ansari

Department of Botany-Herbal Sciences, Birla College, Kalyan-421 304, India

Swapneel P. Koli

Department of Botany-Herbal Sciences, Birla College, Kalyan-421 304, India

Darshana A. Patil

Department of Botany, Smt. Chandibai Himathmal Mansukhani College, Ulhasnagar-421 003, India

Corresponding Author(s) : Avinash G. Patil

dravinashpatil@rediffmail.com

Asian Journal of Chemistry, Vol. 25 No. 7 (2013): Vol 25 Issue 7
Article Published : February 4, 2013

Abstract

Chromatographic techniques can be used to document phytochemical fingerprints and quantitate chemical markers in the herbal raw material. Sufoof-e-mohazzil (SM) is one of the most trusted and sold Unani formulation. Sufoof-e-mohazzil which has been used in Unani Pharmacy since antiquity is used as antiobesity agent, digestive and for controlling high cholesterol levels. Thymol as phytochemical marker was quantified using HPTLC from in-house and two marketed formulations (SM-1 and SM-2) of Sufoof-e-mohazzil. Quantification was carried out on silica gel 60 F₂₅₄ pre-coated HPTLC plates using toluene: ethyl acetate: GAA [97:3:0.2, (v/v/v)] as mobile phase. The response was linear over the range of 1 μg/mL to 5 μg/mL with correlation coefficient of 0.986. Accuracy of the method was checked by recovery studies at three different levels and the average percentage recoveries were found to be 97.94 ± 0.5. The concentration of the thymol was found to be 2.780 μg/mL for in-house formulation whereas 2.180 and 1.768 μg/mL for SM-1 and SM-2 respectively. The method was validated for linearity, precision, accuracy and ruggedness and can be used for routine quality control of Unani formulation Sufoof-e-mohazzil.

Keywords

Sufoof-e-mohazzil HPTLC Thymol Method validation

G. Patil, A., Y. Ansari, A., P. Koli, S., & A. Patil, D. (2013). HPTLC Method Development and Validation of Secondary Metabolite-Thymol from Sufof-E-Mohazzil: A Unani Formulation. Asian Journal of Chemistry, 25(7), 3827–3830. https://doi.org/10.14233/ajchem.2013.13806

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References

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References

A. Husain, G.D. Sofi, Tajuddin, R. Dang and N. Kumar, Med. J. Islamic World Acad. Sci., 18, 27 (2010).

K.H. Mohammad, Bayaz-e-Kabir. Hikmat Book Depot Hyderabad, edn. 15, vol . 2 (1977).

A.H. Gilani and A. Rahman, J. Ethanopharmacol., 100, 43 (2005).

WHO, Traditional Medicine Strategy 2002-2005 World Health Organization; Geneva (2002).

C. Zidorn, B. Schubert and H. Stuppner, Biochem. Syst. Eco., 33, 855 (2005).

T. Layloff, Pharm. Technol., 15, 146 (1991).

WHO, Guideline for the Assessment of Herbal Medicine, Munich, World Health Organization (1991).

Committee for Medicinal Product for Human Use (CHMP), Guidelines for the Quality of Herbal Medicinal Products/Traditional Herbal Medicinal Products. European Medicines Agency Inspections (2005). http://www.emea.eu.int

W.J. Welsh, W.K. Lin, S.H. Tersigni, E. Collantes, R. Duta, M.S. Carey, W.L. Zielinski, J. Brower, J.A. Spencer and T.P. Layloff, Anal. Chem., 68, 3473 (1996).

Y.B. Ji, Q.S. Xu, Y.Z. Hu and Y.V. Heyden, J. Chromatogr. A., 1066, 97 (2005).

A.H. Liu, Y.H. Lin, M. Yang, H. Guo, S.H. Guan, J.H. Sun and D.A. Guo, J. Chromatogr. B, 846, 32 (2007).

S. Shailajan and A. Sharma, Asian J. Plant Sci., 7, 611 (2008).

British Herbal Pharmacopeia. British Herbal Medicine Association (1996).

B.L. Chauhan, S.K. Mitra, A.R. Mohan, S. Gopumadhavan and S.D. Anturlikar, Indian Drugs, 31, 333 (1994).

G. Szepesi, J. Planer Chromatogr. Mod. TLC, 6, 187 (1993).

M. Brigitte, The Desktop Guide to Herbal Medicine. Published by Elsevier, a division of Reed Elsevier (India) Private limited, New Delhi, edn. 1 (2007).

H. Wagner and S. Bladt, Plant Drug Analysis; A Thin Layer Chromatography Atlas. Berlin: Springer (1996).

Z. Zekovic, Z. Lepojevic and D.J. Vujic, Chromatographia, 51, 175 (2000).

H. Hajimehdipoor, M. Shekarchi, M. Khanavi, N. Adib and M. Amri, Pharmacog. Mag., 6, 154 (2010).

British Pharmacopeia, Published by British Government, edn. 5, vol. 2 (2005).

N. Wienkotter, F. Begrow, U. Kinzinger, D. Schierstedt and E.J. Verspohl, Planta Medica, 73, 629 (2007).

Quality Standards of Indian Medicinal Plants, Vol. I, Indian Council of Medical Research, New Delhi, vol. 1, pp.10-50 (2003).

A. Grigore, I.N.A. Paraschiv, S. Colceru-Mihul1, C. Bubueanu, E. Draghici and M. Ichim, Romanian Biotechnol. Lett., 15, 5436 (2010).

Q2A, ICH, Q2A (R1) Validation of Analytical Procedures: Text and Methodology, International Conference on Harmonization, Geneva (2005).

Issue

Vol. 25 No. 7 (2013): Vol 25 Issue 7

HPTLC Method Development and Validation of Secondary Metabolite-Thymol from Sufof-E-Mohazzil: A Unani Formulation

Corresponding Author(s) : Avinash G. Patil

Abstract

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