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Vol. 25 No. 7 (2013): Vol 25 Issue 7

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Spectrophotometric Determination of Hydrogen Peroxide Based on Fading of Tribromoarsenazo Oxidation by Hydrogen Peroxide Catalyzed by Hemoglobin

https://doi.org/10.14233/ajchem.2013.14475C
Qing-Zhou Zhai
Research Center for Nanotechnology, South Campus, Changchun University of Science and Technology, 7186 Weixing Road, Changchun 130022, P.R. China
Xiao-Xia Zhang
Research Center for Nanotechnology, South Campus, Changchun University of Science and Technology, 7186 Weixing Road, Changchun 130022, P.R. China
Rui-Bo Zhang
Research Center for Nanotechnology, South Campus, Changchun University of Science and Technology, 7186 Weixing Road, Changchun 130022, P.R. China

Corresponding Author(s) : Qing-Zhou Zhai

zhaiqingzhou@163.com

Asian Journal of Chemistry, Vol. 25 No. 7 (2013): Vol 25 Issue 7

Abstract

In pH 3.2 CH3COOH-CH3COONa buffer solution, bovine hemoglobin (Hb) catalyzes the decolouring of oxidation tribromoarsenazo (TB-ASA) by hydrogen peroxide and between fading degree DA and H2O2 concentration a linear relationship exists. Based on this principle, a novel method for the determination of H2O2 has been developed. The results showed that the maximum absorption wavelength of the system is 400 nm. At this wavelength, the apparent molar absorptivity of the method is e400 nm = 1.01 × 103 L·mol-1·cm-1 and the linear range is 4.0 × 10-5-3.4 × 10-4 mol L-1 with a detection limit of 2.1 × 10-5 mol L-1. The present method has been successfully applied to the determination of H2O2 in rain waters.

Keywords

Hydrogen peroxide Tribromoarsenazo Catalytic oxidation Bovine hemoglobin Rain water Spectrophotometry
Zhai, Q.-Z., Zhang, X.-X., & Zhang, R.-B. (2013). Spectrophotometric Determination of Hydrogen Peroxide Based on Fading of Tribromoarsenazo Oxidation by Hydrogen Peroxide Catalyzed by Hemoglobin. Asian Journal of Chemistry, 25(7), 4099–4102. https://doi.org/10.14233/ajchem.2013.14475C
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