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Purification Process of Daphnetin from Zushima Herb. by Macroporous Adsorbent Resin
Corresponding Author(s) : Yan Liu
Asian Journal of Chemistry,
Vol. 25 No. 7 (2013): Vol 25 Issue 7
Abstract
To study the conditions and parameters for the purification of daphnetin with macroporous adsorbent resin, the adsorption and desorption of nine macroporous adsorbent resins for daphnetin were compared. The optimal adsorbent was HPD-722. The appropriate adsorption/ desorption conditions were determined. Conditions are as follows: the concentration of daphnetin in extract solution was at 0.384 g/L, pH value of 7 to 8, volume at 20 BV, the extract solution passed through the column at a flow rate of 1 BV/h and room temperature, 65 % alcohol was satisfactory eluant for desorption, the column was washed with 3 BV 65 % alcohol at a flow rate of 2 BV/h. The purity of daphnetin was increased to 70 % or more.
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- M. Deiana, A. Rosa, V. Casu, F. Cottiglia, L. Bonsignore and M.A. Dessi, J. Am. Oil Chem. Soc., 80, 65 (2003).
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- Y. Wang, Z.X. Lu, X.M. Bie and F.X. Lv, Eur. Food Res. Technol., 231, 189 (2010).
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- M. Jie, C.P. Wan, Y.Y. Yu and S.W. Cao, Asian J. Chem., 23, 3964 (2011).
- Y.F. Liu, L. Shan and X.G. Wang, J. Am. Oil Chem. Soc., 86, 183 (2009).
- Z.P. Yin, B.Y. Zhang and H.Y. Chen, Frontiers Agric. China, 5, 637 (2011).
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- L.A. Rodrigues and M.L.C.P. da Silva, Adsorption, 16, 173 (2010).
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References
M. Deiana, A. Rosa, V. Casu, F. Cottiglia, L. Bonsignore and M.A. Dessi, J. Am. Oil Chem. Soc., 80, 65 (2003).
D.A. Egan, C. Duff, L. Jordan, R. Fitzgerald, S. Connolly and G. Finn, Chromatographia, 58, 649 (2003).
D.L. Hua, C.Q. Ma, L.F. Song, S. Lin, Z.B. Zhang, Z.X. Deng and P. Xu, Appl. Microbiol. Biotechnol., 74, 783 (2007).
L.Y. Yao, L.W. Wang, B. Zhao, X.F. Yuan, X.D. Wang, Y.C. Wang, H.M. Su and S.M. Niu, Asian J. Chem., 24, 871 (2012).
Y. Wang, Z.X. Lu, X.M. Bie and F.X. Lv, Eur. Food Res. Technol., 231, 189 (2010).
R.F. Wang, J.L. Yang and Z.Q. Shi, Frontiers Chem. Eng. China, 2, 171 (2008).
Q. Huo and X.M. Gu, Asian J. Chem., 22, 6891 (2010).
M. Jie, C.P. Wan, Y.Y. Yu and S.W. Cao, Asian J. Chem., 23, 3964 (2011).
Y.F. Liu, L. Shan and X.G. Wang, J. Am. Oil Chem. Soc., 86, 183 (2009).
Z.P. Yin, B.Y. Zhang and H.Y. Chen, Frontiers Agric. China, 5, 637 (2011).
V. Tharanitharan and K. Srinivasan, Asian J. Chem., 22, 3036 (2010).
L.A. Rodrigues and M.L.C.P. da Silva, Adsorption, 16, 173 (2010).
S. Haider, Z.X. Li and H. Lin, J. Ocean Univ. China (Eng. Ed.), 8, 425 (2009).
B.Q. Fu, J. Liu, H. Li, L. Li and F.S.C. Lee and X.R. Wang, J. Chromatogr. A, 1089, 18 (2005).
E.M. Silva, D.R. Pompeu and Y. Larondelle, Sep. Purif. Technol., 53, 274 (2007).