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RP-HPLC Method for Simultaneous Estimation of Bromohexine Hydrochloride, Dextromethorphan Hydrobromide and Guaiphenesin in Soft Gel Capsules
Corresponding Author(s) : P.S. Mehta
Asian Journal of Chemistry,
Vol. 25 No. 6 (2013): Vol 25 Issue 6
Abstract
A simple, precise and accurate reverse phase high performance liquid chromatographic method has been developed for simultaneous estimation of guaiphenesin, dextromethorphan hydrobromide and bromohexine hydrochloride in their soft gel formulations. The chromatographic conditions were standardized using Phenomenex Luna C18 (250 mm × 4.6 mm i.d., 5 μm particle size) with UV detection at 258 nm and mobile phase consisting of methanol: 0.05 M phosphate buffer pH 3.0 (60 : 40 v/v). Mobile phase was delivered at the flow rate of 1.3 mL/min and separation was completed within 8 min. The retention times of guaiphenesin, dextromethorphan hydrobromide and bromhexine hydrochloride were 3.1 min, 4.1 min and 6.8 min respectively. Calibration curves were linear with correlation coefficient 0.998. 0.999 and 0.999 over a concentration range of 50-250, 5-25 and 1-6 μg/mL for guaiphenesin, dextromethorphan hydrobromide and bromhexine hydrochloride respectively. Recoveries were between 97.04-100.15, 101.92-104.67 and 102.01-102.55 respectively. The proposed method was validated and successfully applied to the estimation of three drugs in combined soft gel formulation.
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- British Pharmacopoeia, London: The Stationery Office, vol. 1, 280, 632 and 976 (2009).
- Indian Pharmacopoeia, Indian Pharmacopeoia Comission Ghaziabad, vol. 1 and 2, p. 195, 395 and 559 (2007).
- United States Pharmacopoiea, USP32, NF27, The Official Compendia of Standards, Vol. 1, pp. 2106 and 2533 (2009).
- A.R. Lee and T.M. Hu, J. Pharm. Biomed. Anal., 12, 747 (1994).
- S. Gangwal and P. Trivedi, Indian J. Pharm. Sci., 60, 238 (1998).
- M. Dhoka, V. Gawande and P. Joshi, Int. J. Pharm. Pharmaceut. Sci., 2, 129 (2010).
- B. Jayalakshmi, J. Ramesh, T.N. Kalpana and R. Vijayamirtharaj, J. Pharm. Res., 3, 2868 (2010).
- W.O. McSharry and I.V. Savage, J. Pharm. Sci., 69, 212 (1980).
- S.M. Amer and M. Shehata, J. AOAC Int., 91, 276 (2002).
- V.S. Lalit and B.B. Sanjaykumar, Pharm. Anal. Acta, 1, 1 (2010).
- M.S. Bhatia, S.G. Kaskhedikar and S.C. Chaturvedi, Indian J. Pharm. Sci., 62, 61 (2000).
- M.S. Bhatia, S.G. Kaskhedikar and S.C. Chaturvedi, Indian Drugs, 36, 702 (1999).
- T. Mingchen and J.R. Pacifico, J. Chromatogr. Sci., 26, 636 (1988).
- V.V. Vaidya, M. Khanolkar and J.N. Gadre, Indian Drugs, 38, 16 (2001).
- V. Galli and C. Barbas, J. Chromatogr. A, 1048, 207 (2004).
References
British Pharmacopoeia, London: The Stationery Office, vol. 1, 280, 632 and 976 (2009).
Indian Pharmacopoeia, Indian Pharmacopeoia Comission Ghaziabad, vol. 1 and 2, p. 195, 395 and 559 (2007).
United States Pharmacopoiea, USP32, NF27, The Official Compendia of Standards, Vol. 1, pp. 2106 and 2533 (2009).
A.R. Lee and T.M. Hu, J. Pharm. Biomed. Anal., 12, 747 (1994).
S. Gangwal and P. Trivedi, Indian J. Pharm. Sci., 60, 238 (1998).
M. Dhoka, V. Gawande and P. Joshi, Int. J. Pharm. Pharmaceut. Sci., 2, 129 (2010).
B. Jayalakshmi, J. Ramesh, T.N. Kalpana and R. Vijayamirtharaj, J. Pharm. Res., 3, 2868 (2010).
W.O. McSharry and I.V. Savage, J. Pharm. Sci., 69, 212 (1980).
S.M. Amer and M. Shehata, J. AOAC Int., 91, 276 (2002).
V.S. Lalit and B.B. Sanjaykumar, Pharm. Anal. Acta, 1, 1 (2010).
M.S. Bhatia, S.G. Kaskhedikar and S.C. Chaturvedi, Indian J. Pharm. Sci., 62, 61 (2000).
M.S. Bhatia, S.G. Kaskhedikar and S.C. Chaturvedi, Indian Drugs, 36, 702 (1999).
T. Mingchen and J.R. Pacifico, J. Chromatogr. Sci., 26, 636 (1988).
V.V. Vaidya, M. Khanolkar and J.N. Gadre, Indian Drugs, 38, 16 (2001).
V. Galli and C. Barbas, J. Chromatogr. A, 1048, 207 (2004).