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Vol. 25 No. 6 (2013): Vol 25 Issue 6

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Self-Assembled Human IgG Monolayers on Mixed Thiols Modified Gold Surfaces

https://doi.org/10.14233/ajchem.2013.13604
Zhengjian Lv
Key Laboratory of Biorheological Science and Technology, Ministry of Education and Institute of Biochemistry and Biophysics, College of Bioengineering, Chongqing University, Chongqing 400044, P.R. China
Jianhua Wang
Key Laboratory of Biorheological Science and Technology, Ministry of Education and Institute of Biochemistry and Biophysics, College of Bioengineering, Chongqing University, Chongqing 400044, P.R. China
Guoping Chen
Key Laboratory of Biorheological Science and Technology, Ministry of Education and Institute of Biochemistry and Biophysics, College of Bioengineering, Chongqing University, Chongqing 400044, P.R. China

Corresponding Author(s) : Jianhua Wang

wjh@cqu.edu.cn; cqubio@hotmail.com

Asian Journal of Chemistry, Vol. 25 No. 6 (2013): Vol 25 Issue 6

Abstract

The human IgG protein monolayers were fabricated on mixed thiols modified gold substrates by self-assembled monolayers method. The surface properties of protein monolayers were characterized by contact angle measurements, X-ray photoelectron spectroscopy and atomic force microscopy, respectively. It was found that the contact angles of the protein layers on the mixed thiols films containing 20 %, 40 %, 60 %, 80 %, 100 % of 16-mercaptohexadecanoic acid were 83.5 ± 3.3º, 56.7 ± 3.1º, 38.6 ± 2.9º, 25.8 ± 2.6º, 14.2 ± 2.3º, respectively. The atomic concentration of nitrogen of protein layers on pure 16-mercaptohexadecanoic acid film was counted to be 11.43 ± 0.56 %. Depth profile analysis showed the protein layer and 16-mercaptohexadecanoic acid film have the thickness of 7 nm and 2 nm, respectively. Furthermore, the adhesive forces between antibody and antigen layers with molar ratios of 16-mercaptohexadecanoic acid of 0, 0.2, 0.4, 0.6, 0.8 and 1.0 were determined to be 11 ± 2, 158 ± 21, 325 ± 24, 589 ± 36, 814 ± 41, 883 ± 45 pN, respectively. In summary, these results suggested that the human IgG protein monolayers were successfully formed and the surface coverage of protein molecules increased parallel with the increasing of 16-mercaptohexadecanoic acid content. Thus, they demonstrated that the mixed thiols based self-assembled monolayers method is a reliable technique for fabricating protein monolayers with adjustable surface coverage and good controllability.

Keywords

Human IgG Self assembled monolayers Contact angle X-ray photoelectron spectroscopy Atomic force microscopy
Lv, Z., Wang, J., & Chen, G. (2013). Self-Assembled Human IgG Monolayers on Mixed Thiols Modified Gold Surfaces. Asian Journal of Chemistry, 25(6), 3237–3243. https://doi.org/10.14233/ajchem.2013.13604
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