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Characterization of Chitosanase from Indralaya Swamp Bacteria, South Sumatera, Indonesia
Corresponding Author(s) : Ace Baehaki
Asian Journal of Chemistry,
Vol. 25 No. 6 (2013): Vol 25 Issue 6
Abstract
The extracellular chitosanase-production capacity of 14 bacterial isolates taken from Indralaya Swamp South Sumatera, was evaluated in liquid cultures. The GB2 isolate was selected due to its high chitosanase activity. The optimum pH and temperature of chitosanase produced by GB2 were 8 and 30 ºC, respectively. Ca2+ and Fe2+ increased GB2 chitosanase whereas Na+ and K+ inhibited the enzyme. Study on the effect of metals ion indicated that chitosanase GB2 was metaloenzyme. Moleculer weights were determined by using SDS-PAGE and zymogram technique. Moleculer weight of chitosanase isolate GB2 was 36-110 kD.
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- C.L. Chiang, C.T. Chang and H.Y. Sung, Enzyme Microb. Tech., 32, 260 (2003).
- W.J. Jung, J.H. Kuk, K.Y. Kim, K.C. Jung and R.D. Park, Protein Expr. Purif., 45, 125 (2006).
- D. Somashekar and R. Joseph, Bioresour. Technol., 55, 35 (1996).
- H.G. Yoon, H.Y. Kim, H.K. Kim, D.H. Shin, B.S. Hong and H.Y. Cho, Appl. Environ. Microbiol., 66, 3727 (2000).
- Y. Uchida and A. Ohtakara, Methods Enzymol., 161, 501 (1998).
- M. Guttenberger, in ed.: J.E. Celis, Protein Determination, Cell Biology: A Laboratory Manual, Academic Press, vol. 3 (1994).
- D.M. Bollag and Edelstein, Protein Methods, New York: Wiley-Liss (1991).
- J. Grenier, J. Trudel and A. Asselin, in eds.: R.A.A. Muzzarelli and M.G. Peter, Gel Electroforetic Analysis of Chitinase, Chitosanase and Chitin Deacetylase, Chitin Handbook. Italy:Atec, Grottammare (1997).
- K.D. Splinder and C. Rapp, in eds.: R.A.A. Muzzarelli and M.G. Peter, Detection of Chitin Degradating Enzyme on Gels, Chitin Handbook. Italy: Atec, Grottammare (1997).
- J.K. Park, K. Shimono, N. Ochiai, K. Shigeru, M. Kurita, Y. Ohta, K. Tanaka, H. Matsuda and M. Kawamukai, J. Bacteriol., 181, 6642 (1999).
- M.E. Mahata, Y.C. Soo,A. Dharma, I. Ryanto, Y. Rizal and M. Kawamukai, J. Mikrobiol. Indones, 10, 21 (2005).
- H. Kimoto, H. Kusaoke, I. Yamamoto, Y. Fujii, T. Onodera and A. Taketo, J. Biol. Chem., 277, 14695 (2002).
- K. Shimono, K. Shigeru, A. Tsuchiya, N. Itou, Y. Ohta, K. Tanaka, T. Nakagawa, H. Matsuda and M. Kawamukai, J. Biochem., 131, 87 (2002).
- J.P. Glusker, in ed.: G. Allen, The Binding of Ions to Proteins. Protein: A Comprehensive Treatise, Stanford: JAI Press, vol. 2 (1999).
References
C.L. Chiang, C.T. Chang and H.Y. Sung, Enzyme Microb. Tech., 32, 260 (2003).
W.J. Jung, J.H. Kuk, K.Y. Kim, K.C. Jung and R.D. Park, Protein Expr. Purif., 45, 125 (2006).
D. Somashekar and R. Joseph, Bioresour. Technol., 55, 35 (1996).
H.G. Yoon, H.Y. Kim, H.K. Kim, D.H. Shin, B.S. Hong and H.Y. Cho, Appl. Environ. Microbiol., 66, 3727 (2000).
Y. Uchida and A. Ohtakara, Methods Enzymol., 161, 501 (1998).
M. Guttenberger, in ed.: J.E. Celis, Protein Determination, Cell Biology: A Laboratory Manual, Academic Press, vol. 3 (1994).
D.M. Bollag and Edelstein, Protein Methods, New York: Wiley-Liss (1991).
J. Grenier, J. Trudel and A. Asselin, in eds.: R.A.A. Muzzarelli and M.G. Peter, Gel Electroforetic Analysis of Chitinase, Chitosanase and Chitin Deacetylase, Chitin Handbook. Italy:Atec, Grottammare (1997).
K.D. Splinder and C. Rapp, in eds.: R.A.A. Muzzarelli and M.G. Peter, Detection of Chitin Degradating Enzyme on Gels, Chitin Handbook. Italy: Atec, Grottammare (1997).
J.K. Park, K. Shimono, N. Ochiai, K. Shigeru, M. Kurita, Y. Ohta, K. Tanaka, H. Matsuda and M. Kawamukai, J. Bacteriol., 181, 6642 (1999).
M.E. Mahata, Y.C. Soo,A. Dharma, I. Ryanto, Y. Rizal and M. Kawamukai, J. Mikrobiol. Indones, 10, 21 (2005).
H. Kimoto, H. Kusaoke, I. Yamamoto, Y. Fujii, T. Onodera and A. Taketo, J. Biol. Chem., 277, 14695 (2002).
K. Shimono, K. Shigeru, A. Tsuchiya, N. Itou, Y. Ohta, K. Tanaka, T. Nakagawa, H. Matsuda and M. Kawamukai, J. Biochem., 131, 87 (2002).
J.P. Glusker, in ed.: G. Allen, The Binding of Ions to Proteins. Protein: A Comprehensive Treatise, Stanford: JAI Press, vol. 2 (1999).