HPLC Fingerprint of Solid-State Fermentation of Fugui Gutong Compound Prescription

Jianshu Su; Pingfang Tian; Qiang Lin; Baining Liu; Laili Zheng; Xizhen Ge

doi:10.14233/ajchem.2013.13518

Issue

Vol. 25 No. 6 (2013): Vol 25 Issue 6

Issue Published : December 26, 2012

This work is licensed under a Creative Commons Attribution 4.0 International License.

HPLC Fingerprint of Solid-State Fermentation of Fugui Gutong Compound Prescription

https://doi.org/10.14233/ajchem.2013.13518

Jianshu Su

Biochemical Engineering College, Beijing Union University, Beijing 100023, P.R. China ; College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, P.R. China

Pingfang Tian

College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, P.R. China

Qiang Lin

Biochemical Engineering College, Beijing Union University, Beijing 100023, P.R. China

Baining Liu

Biochemical Engineering College, Beijing Union University, Beijing 100023, P.R. China

Laili Zheng

Biochemical Engineering College, Beijing Union University, Beijing 100023, P.R. China ; College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, P.R. China

Xizhen Ge

Biochemical Engineering College, Beijing Union University, Beijing 100023, P.R. China

Corresponding Author(s) : Xizhen Ge

gexizhen@163.com

Asian Journal of Chemistry, Vol. 25 No. 6 (2013): Vol 25 Issue 6
Article Published : January 8, 2013

Abstract

Aspergillus niger (E6.22) was employed for solid state fermentation of Fu-Gui-Gu-Tong (FGGT, hereafter solid state fermentation FGGT), a notable Chinese compound prescription, to attenuate its toxicity. Results indicated that the optimal spore density was 1.8 × 10⁴ mL^-1, incubation time was 7d, pH was 6.5, incubation temperature was 30 ºC. Under these conditions, paeoniflorin and total alkaloids were increased, but aconitine was decreased. Additionally, high-performance liquid chromatographic fingerprint of solid state fermentation Fu-Gui-Gu-Tong were determined and applied for monitoring the fermentation stability. The similarity index of 10 samples was 0.978, suggesting that the procedure of solid state fermentation Fu-Gui-Gu-Tong was stable in different batches. To investigate its acute toxicity, the mice was orally administered with graded doses of solid state fermentation Fu-Gui-Gu-Tong extract, the LD₅₀ of the solid state fermentation Fu-Gui-Gu-Tong group was higher than Fu-Gui-Gu-Tong, meaning the less toxicity of solid state fermentation Fu-Gui-Gu-Tong. Hence, it is concluded that A. niger (E6.22) could alleviate the toxicity of Fu-Gui-Gu-Tong through solid state fermentation and the fermentation procedure was reliable and applicable.

Keywords

Aspergillus niger Fu-Gui-Gu-Tong Solid state fermentation HPLC fingerprint

Su, J., Tian, P., Lin, Q., Liu, B., Zheng, L., & Ge, X. (2013). HPLC Fingerprint of Solid-State Fermentation of Fugui Gutong Compound Prescription. Asian Journal of Chemistry, 25(6), 3063–3067. https://doi.org/10.14233/ajchem.2013.13518

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K. Wada, M. Nihira and Y. Ohno, J. Ethnopharmacol., 105, 89 (2006).

K. Wada, M. Nihira, H. Hayakawa, Y. Tomita, M. Hayashida and Y. Ohno, Forensic. Sci. Int., 148, 21 (2005).

W.L. Liu, Z.Q. Liu, F.R. Song and S.Y. Liu, Chem. J. Chineses Univ., 32, 717 (2011).

J.S. Su, B.N. Liu, P.F. Tian, Q. Lin, Y.X. Zhao and X.Z. Ge, J. Beijing Univ. Chem. Technol., 37, 97 (2010).

L.P. Zhu and Z.P. Xu, Chin. Traditional Pat. Med., 27, 8202 (2005).

A. Sharma, V. Vivekanand and R.P. Singh, Bioresour. Technol., 99, 3444 (2008).

R.R. Singhaniaa, A.K. Patel, C.R. Soccolc and A. Pandeya, Biochem. Eng. J., 44,13 (2009).

L.L. Zheng, P.F. Tian, Y.S. Li, X.Y. Qiao, R. Wang and X.Z. Ge, J. Beijing Univ. Chem. Technol., 36, 80 (2009).

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Q. Chen, Method Study of Traditional Chinese Medicine Pharmacology, People's Health Press, Beijing (1993).

A.B. Diaz, I. Caro, Ory. I. De and A. Blandino, Enzym. Microb. Technol., 41, 302 (2007).

Y. Ohno, J. Toxicol. Toxin. Rev., 17, 1 (1998).

T.Y.K. Chan, Hum. Exp. Toxicol., 30, 2023 (2011).

M.W. Carey, N.V. Rao, B.R. Kumar and G.K. Mohan, J. Ethnopharmacol., 130, 179 (2010).

Issue

Vol. 25 No. 6 (2013): Vol 25 Issue 6

HPLC Fingerprint of Solid-State Fermentation of Fugui Gutong Compound Prescription

Corresponding Author(s) : Xizhen Ge

Abstract

Keywords

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