Spectrophotometric Determination of Cefixime in Pure Form and in Syrian Pharmaceuticals Through Complexation with Cu(II)

Abstract

Spectroscopic analytical study for the determination of cefixime in pure and its Syrian pharmaceutical formations through complexation with Cu(II) in acetate buffer at pH = 7.8 has been developed. The method is based on the formation pink colour complex between cefixime and Cu(II). The maximum absorbance of the coloured complex occurred at l = 546 nm and the molar absorptivity is 3.28 × 10³ L mol^-1 cm^-1. The reaction conditions have been optimized to obtain the complex. Under optimum conditions the absorbance of complex was found to increase linearly with increase in concentrations of cefixime, which corroborated with the correlation coefficient values (1:1). The linear range of the calibration curve was 0.453-9.069 μg mL^-1 with correlation coefficients = 0.9975 in all cases. Overall recoveries were of the order of 98.00-101.50 %. The limit of detection and limit of quantification was found to be 0.075 μg mL^-1 and 0.22 μg mL^-1, respectively. The proposed method was simple, economic, accurate and successfully applied to the determination of cefixime in Syrian pharmaceuticals, the results obtained agree well with the contents stated on the labels.