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Vol. 25 No. 5 (2013): Vol 25 Issue 5

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Electron-Transfer Reaction of Cytochrome c Adsorbed on Mixed Alkanethiol Monolayer Electrode and Its Electrocatalytic Activity

https://doi.org/10.14233/ajchem.2013.13450
Yu Wang
College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang 330022, P.R. China
Lingli Wan
College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang 330022, P.R. China
Yonghai Song
College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang 330022, P.R. China
Li Wang
College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang 330022, P.R. China

Corresponding Author(s) : Yonghai Song

yhsong@jxnu.edu.cn

Asian Journal of Chemistry, Vol. 25 No. 5 (2013): Vol 25 Issue 5

Abstract

The electron-transfer kinetics of cytochrome c (cyt. c) immobilized on self-assembled monolayers modified gold electrode was demonstrated. Mixed monolayer films of 6-mercapto-1-hexanol and 11-mercaptoundecanoic acid are used to adsorb cytochrome c on the surface of gold electrode. These adsorbed cytochrome c molecules remained a dilute concentration on the mixed films. Electrochemical method and scanning tunneling microscopy technique was used to study the adsorbed protein. It showed a surface-controlled electrode process with the electron transfer rate constant of 65 s-1. The adsorbed cytochrome c maintained its activity and could also electrocatalyze the reduction of hydrogen peroxide, the Kmapp for this sensor was found to be 3.41 mM.

Keywords

Cytochrome c Self-assembled monolayers Direct electrochemistry Electrocatalyze
Wang, Y., Wan, L., Song, Y., & Wang, L. (2012). Electron-Transfer Reaction of Cytochrome c Adsorbed on Mixed Alkanethiol Monolayer Electrode and Its Electrocatalytic Activity. Asian Journal of Chemistry, 25(5), 2547–2551. https://doi.org/10.14233/ajchem.2013.13450
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References
  1. S.D. Varfolomeev and I.V. Berezin, Abstracts of Paper, Advances in Physical Chemistry: Current Developments in Electrochemistry and Corrosion, Moscow: MIR, p. 60 (1982).
  2. N.K. Chaki and K. Vijayamohanan, Biosens. Bioelectron., 17, 1 (2002).
  3. M. Fedurco, Coord. Chem. Rev., 209, 263 (2000).
  4. C. Hinnen, R. Parsons and K. Nikki, J. Electroanal. Chem., 147, 329 (1983).
  5. D.E. Reed and F.M. Hawkridge, Anal. Chem., 59, 2334 (1987).
  6. F.A. Armstrong, Struct. Bonding, 72, 137 (1990).
  7. L. Wang and E.K. Wang, Electrochem. Commun., 6, 49 (2004).
  8. C.H. Lei, F. Lisdat, U. Wollenberger and F.W. Scheller, Electroanal., 11, 274 (1999).
  9. H. Ju, S. Liu, B. Ge, F. Lisdat and F.W. Scheller, Electroanal., 14, 141 (2002).
  10. P.M. Allen, H.A.O. Hill and N.J. Walton, J. Electroanal. Chem., 178, 69 (1984).
  11. G. McLendon, Acc. Chem. Res., 21, 160 (1988).
  12. M.J. Tarlov and E.F. Bowden, J. Am. Chem. Soc., 113, 1847 (1991).
  13. M. Collinson, E.F. Bowden and M.J. Tarlov, Langmuir, 8, 1247 (1992).
  14. S. Song, R.A. Clark, E.F. Bowden and M.J. Tarlov, J. Phys. Chem. B, 97, 6564 (1993).
  15. Z.Q. Feng, S. Imabayashi, T. Kakiuchi and K.J. Niki, J. Chem. Soc. Faraday Trans., 93, 1367 (1997).
  16. A.E. Kasmi, J.M. Wallace, E.F. Bowden, S.M. Binet and R.J. Linderman, J. Am. Chem. Soc., 120, 225 (1998).
  17. A. Avilla, B.W. Gregory, K. Niki and T.M. Cotton, J. Phys. Chem. B, 104, 2759 (2000).
  18. L. Wang and D.H. Waldeck, J. Phys. Chem. C, 112, 1351 (2008).
  19. H. Yamamoto, H. Liu and D.H. Waldeck, Chem. Commun., 1032 (2001).
  20. J. Wei, H. Liu, A.R. Dick, H. Yamamoto, Y. He and D.H. Waideck, J. Am. Chem. Soc., 124, 9591 (2002).
  21. E. Sabatani, I. Rubinstein, R. Maoz and J. Sagiv, J. Electroanal. Chem., 219, 365 (1987).
  22. J. Clavilier, R. Faure, G. Guinet and R. Durand, J. Electroanal. Chem., 107, 205 (1980).
  23. H.O. Finklea, Electroanalytical Chemistry, New York: Marcel Dekker (1996).
  24. A.J. Bard and L.R. Faulkner, Electrochemical Methods: Fundamentals and Applications, New York: Wiley (1980).
  25. C.J. Miller, Physical Electrochemistry: Principles, Methods and Applications, New York: Marcel Dekker, p. 27 (1995).
  26. H.O. Finklea, D.A. Snider, J. Fedyk, E. Sabatani, Y. Gafni and I. Rubinstein, Langmuir, 9, 3660 (1993).
  27. G.E. Poirier, Chem. Rev., 97, 1117 (1997).
  28. R.A. Scott, Cytochrome C:A Multidisciplinary Approach, Sausalito (1996).
  29. J.E.T. Anderson, P. Møller, M.V. Pedersen and J. Ulstrup, Surf. Sci., 325, 193 (1995).
  30. D. Hobara, S. Imabayashi and T. Kakiuchi, Nano Lett., 2, 1021 (2002).
  31. X.L. Yuan, F.M. Hawkridge and J.F. Chlebowski, J. Electroanal. Chem., 350, 29 (1993).
  32. G. Battistuzzi, M. Borsari, M. Sola and F. Francia, Biochemistry, 36, 16247 (1997).
  33. J. Sun, J.F. Wishart, R.V. Eldik, R.D. Shalderes and T.W. Swaddle, J. Am. Chem. Soc., 117, 2600 (1995).
  34. H. Allen, O. Hill, N.I. Hunt and A.M. Bond, J. Electroanal. Chem., 436, 17 (1997).
  35. A. Szucs and M. Novak, J. Electroanal. Chem., 383, 75 (1995).
  36. X. Chen, R. Ferrigno, J. Yang and G.M. Whitesides, Langmuir, 18, 7009 (2002).
  37. R.W. Murray, Electroanalytical Chemistry, New York: Marcel Dekker, p. 191 (1984).
  38. L. Tender, M.T. Carter and R.W. Murray, Anal. Chem., 66, 3173 (1994).
  39. K. Weber and S.E. Creager, Anal. Chem., 66, 3164 (1994).
  40. E. Laviron, J. Electroanal. Chem., 101, 19 (1979).
  41. R.A. Kamin and G.S. Willson, Anal. Chem., 52, 1198 (1980).
  42. J. Li, S.N. Tan and H. Ge, Anal. Chim. Acta, 335, 137 (1996).
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