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Vol. 25 No. 5 (2013): Vol 25 Issue 5

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Assessment of Aflatoxin M1 Levels by Enzyme-linked Immunosorbent Assay in Yoghurt Consumed in Tehran, Iran

https://doi.org/10.14233/ajchem.2013.14058
Hamid Reza Tavakoli
Department of Nutrition, Health Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Abolfazl Kamkar
Department of Food Hygiene, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Majid Riazipour
Department of Microbiology, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran
Amir Sasan Mozaffari Nejad
Center for Biotechnology, institute of Science and Technology, Jawaharlal Nehru Technological University, Hyderabad-500 085, India ; Young Researchers Club, Garmsar Branch, Islamic Azad University, Garmsar, Iran
Hassan Rafati Shaldehi
Department of Epidemiology and Statistic, Troma Research Center, Baqiyatallah University of Medical Science, Tehran, Iran

Corresponding Author(s) : Amir Sasan Mozaffari Nejad

asmozafarinejad@yahoo.in

Asian Journal of Chemistry, Vol. 25 No. 5 (2013): Vol 25 Issue 5

Abstract

Due to their nutritional value, milk products are popular in Iran. However, aflatoxin M1 (AFM1) in milk and dairy products is considered to pose certain hygienic risks for human health. This study was undertaken to determine the occurrence of aflatoxin M1 in 50 yoghurt samples from 2 dairy factories in summer 2008 and winter 2009. Enzyme-linked immunosorbent assay (ELISA) technique was used for the analysis of yoghurt samples. Aflatoxin M1 was found 70 % in the yoghurt samples, ranging from 21.1 to 137.6 ng/kg. Toxin level is 17.14 % of the samples exceeded the Iranian national standard limit i.e. 50 ng/kg. Also, statistically significant seasonal effect was found for yoghurt samples. The result showed that the contamination of the yoghurt in such a level could be a serious public health problem.

Keywords

Aflatoxin M1 Yoghurt Seasonal effect Enzyme-linked immunosorbent assay Iran
Reza Tavakoli, H., Kamkar, A., Riazipour, M., Sasan Mozaffari Nejad, A., & Rafati Shaldehi, H. (2012). Assessment of Aflatoxin M1 Levels by Enzyme-linked Immunosorbent Assay in Yoghurt Consumed in Tehran, Iran. Asian Journal of Chemistry, 25(5), 2836–2838. https://doi.org/10.14233/ajchem.2013.14058
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