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Comparative Analysis of in vitro Antioxidant Activity of Two Selected Plants With a Reference to Antidiabetic Profile
Corresponding Author(s) : Dilipkumar Pal
Asian Journal of Chemistry,
Vol. 25 No. 4 (2013): Vol 25 Issue 4
Abstract
The antioxidant and antidiabetic activity of aerial parts of Aerva sanguinolenta (L) Blume and leaves of Mikania scandens (L.) Willd were investigated by three in vitro and one in vivo assays i.e., by estimating degree of non-enzymatic haemoglobin glycosylation measured colorimetrically at 520 nm; by assaying DPPH free radical scavenging activity; by reduction of phosphomolybdenum complex and by estimating blood glucose levels in alloxan-induced diabetic mice. From the analysis, it was found that the ethyl acetate and chloroform extract of both aerial parts of A. sanguinolenta and leaves of Mikania scandens had higher antioxidant potential than that of other extracts of the plant(s). Among the examined plants, it was also observed that M. scandens L. extracts had better antioxidant and antihyperglycemic action than that of A. sanguinolenta. The antioxidant activity of the extracts was close, identical in magnitude, concentration dependent and comparable to that of standard antioxidant compounds used. Flavonoids and tannins present in the selected extracts may be responsible for such antioxidant and antidiabetic activities.
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- P.M. Basha and N. Madhusudhan, Biologia, 66, 187 (2011).
- B. Halliwell, J.M.C. Gutteridge and C.E. Cross, J. Lab. Clin. Med. 119, 598 (1992).
- M.B. Grisham and J.M. McCord, In ed.: A.E. Teylor, Chemistry and Cytotoxicities of Reactive Oxygen Metabolites; Biology of Oxygen Radicals; American Physiological Society, Bethesda, p. 1 (1986).
- M. Kumar, A. Ahmad, P. Rawat and M.F. Khan, Fitoterapia, 81, 234 (2010).
- R. Mahesh, H.W. Jung, J.H. Park and Y.K. Park, E-J. Chem., 8, 1451 (2011).
- D.K. Pal, P. Maity and K. Samanta, Asian J. Chem., 22, 8246 (2010).
- D.K. Pal, M. Kumar, P. Chakraborty and S. Kumar, Asian J. Chem., 20, 2479 (2008).
- S. Sannigrahi, U.K. Mazumder, A. Mandal, D.K. Pal, S.L. Mishra and S. Roy, Nat. Prod. Commun., 5, 1239 (2010).
- S. Sannigrahi, U.K. Mazumder, D.K. Pal, S. Parida and S. Jain, Iran. J. Pharm. Res., 9, 75 (2009).
- J.R.S. Tabuti, K.A. Lye and S.S. Dhillion, J. Ethnopharmacol., 88, 19 (2003).
- M.M. Alam, S.A. Alam, N.S. Mohammed, N.S. Juyena and M.A. Hashim, Pakistan J. Biol. Sci., 8, 740 (2005).
- S.B. Kosalge and R.A. Fursule, J. Ethnopharmacol., 121, 456 (2009).
- J. Buragohain, Fitoterapia, 79, 388 (2008).
- M.C. Ysrael and K.D. Croft, Planta Med., 56, 268 (1990).
- A. Bishayee and M. Chatterjee, Planta Med., 60, 110 (1994).
- M.A. Mosaddik and K.M. Alam, J. Pharm. Pharmacol., 52, 1157 (2000).
- P. Rajyalakshmi, K.Venkatalaxmi, K. Venkatalakshmamma, Y. Jyothsna, K.B. Devi and V. Suneetha, Plant Foods Human Nutri., 56, 225 (2001).
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- B. Dinda, B. Ghosh, S. Arima, N. Sato and Y. Harigaya, Indian J. Chem., 42, 2223 (2004).
- T. Nakamura, Y. Goda, S. Sakai, K. Kanda, H. Akiyama and M. Toyoda, Phytochemistry, 49, 2097 (1998).
- D.K. Pal, K. Samanta and P. Maity, Asian J. Chem., 22, 2459 (2010).
- S.B.Yadav, V. Tripathy, R.K. Singh and H.P. Pandey, Indian J. Pharm. Sci., 6, 477 (2000).
- D.K. Pal and S.B. Nimse, Asian J. Chem., 18, 3004 (2006).
- W. Pothitirat, M.T. Chomnawang, R. Supabphol and W. Gritsanapan, Fitoterapia, 80, 442 (2009).
- A. Matkowski and M. Piotrowska, Fitoterapia, 77, 346 (2006).
- P.R. Menezes, E.A. Schwarz and C.A.M. Santos, Fitoterapia, 75, 398 (2004).
- S. Badole, N. Patel, S. Bodhankari, B. Jain and S. Bhardwaj, Indian J. Pharmacol., 38, 49 (2006).
- J.S. Dunn and N.G. Mc Letchie, Lancet, 2, 384 (1943).
- M. Aslan, N. Orhan, D.D. Orhan and F. Ergun, J. Ethnopharmacol., 128, 384 (2010).
- D. Mantle, F. Eddeb and A.T. Pickering. J. Ethnopharmacol., 72, 47 (2000).
- O. Firuzi, K. Javidnia, M. Gholami, M. Soltani and R. Miri, Iran. Nat. Prod. Commun., 5, 261 (2010).
- T. Malliar, J. Drobna, J. Kraic, M. Maliarova and J. Jurovata, Biologia, 66, 96 (2011).
- M.R. Vanu, S. Palanivelu and S. Panchanatham, Biol. Pharm. Bull., 29, 693 (2006).
- A. Ozkan, A. Erdogan, M. Sokmen, S. Tugrulay and O. Unal, Biologia, 65, 990 (2010).
References
P.M. Basha and N. Madhusudhan, Biologia, 66, 187 (2011).
B. Halliwell, J.M.C. Gutteridge and C.E. Cross, J. Lab. Clin. Med. 119, 598 (1992).
M.B. Grisham and J.M. McCord, In ed.: A.E. Teylor, Chemistry and Cytotoxicities of Reactive Oxygen Metabolites; Biology of Oxygen Radicals; American Physiological Society, Bethesda, p. 1 (1986).
M. Kumar, A. Ahmad, P. Rawat and M.F. Khan, Fitoterapia, 81, 234 (2010).
R. Mahesh, H.W. Jung, J.H. Park and Y.K. Park, E-J. Chem., 8, 1451 (2011).
D.K. Pal, P. Maity and K. Samanta, Asian J. Chem., 22, 8246 (2010).
D.K. Pal, M. Kumar, P. Chakraborty and S. Kumar, Asian J. Chem., 20, 2479 (2008).
S. Sannigrahi, U.K. Mazumder, A. Mandal, D.K. Pal, S.L. Mishra and S. Roy, Nat. Prod. Commun., 5, 1239 (2010).
S. Sannigrahi, U.K. Mazumder, D.K. Pal, S. Parida and S. Jain, Iran. J. Pharm. Res., 9, 75 (2009).
J.R.S. Tabuti, K.A. Lye and S.S. Dhillion, J. Ethnopharmacol., 88, 19 (2003).
M.M. Alam, S.A. Alam, N.S. Mohammed, N.S. Juyena and M.A. Hashim, Pakistan J. Biol. Sci., 8, 740 (2005).
S.B. Kosalge and R.A. Fursule, J. Ethnopharmacol., 121, 456 (2009).
J. Buragohain, Fitoterapia, 79, 388 (2008).
M.C. Ysrael and K.D. Croft, Planta Med., 56, 268 (1990).
A. Bishayee and M. Chatterjee, Planta Med., 60, 110 (1994).
M.A. Mosaddik and K.M. Alam, J. Pharm. Pharmacol., 52, 1157 (2000).
P. Rajyalakshmi, K.Venkatalaxmi, K. Venkatalakshmamma, Y. Jyothsna, K.B. Devi and V. Suneetha, Plant Foods Human Nutri., 56, 225 (2001).
A. Ghani, Medicinal Plants of Bangladesh, Dhaka: The Asiatic Society of Bangladesh; edn. 2, p. 603 (2003).
B. Dinda, B. Ghosh, S. Arima, N. Sato and Y. Harigaya, Indian J. Chem., 42, 2223 (2004).
T. Nakamura, Y. Goda, S. Sakai, K. Kanda, H. Akiyama and M. Toyoda, Phytochemistry, 49, 2097 (1998).
D.K. Pal, K. Samanta and P. Maity, Asian J. Chem., 22, 2459 (2010).
S.B.Yadav, V. Tripathy, R.K. Singh and H.P. Pandey, Indian J. Pharm. Sci., 6, 477 (2000).
D.K. Pal and S.B. Nimse, Asian J. Chem., 18, 3004 (2006).
W. Pothitirat, M.T. Chomnawang, R. Supabphol and W. Gritsanapan, Fitoterapia, 80, 442 (2009).
A. Matkowski and M. Piotrowska, Fitoterapia, 77, 346 (2006).
P.R. Menezes, E.A. Schwarz and C.A.M. Santos, Fitoterapia, 75, 398 (2004).
S. Badole, N. Patel, S. Bodhankari, B. Jain and S. Bhardwaj, Indian J. Pharmacol., 38, 49 (2006).
J.S. Dunn and N.G. Mc Letchie, Lancet, 2, 384 (1943).
M. Aslan, N. Orhan, D.D. Orhan and F. Ergun, J. Ethnopharmacol., 128, 384 (2010).
D. Mantle, F. Eddeb and A.T. Pickering. J. Ethnopharmacol., 72, 47 (2000).
O. Firuzi, K. Javidnia, M. Gholami, M. Soltani and R. Miri, Iran. Nat. Prod. Commun., 5, 261 (2010).
T. Malliar, J. Drobna, J. Kraic, M. Maliarova and J. Jurovata, Biologia, 66, 96 (2011).
M.R. Vanu, S. Palanivelu and S. Panchanatham, Biol. Pharm. Bull., 29, 693 (2006).
A. Ozkan, A. Erdogan, M. Sokmen, S. Tugrulay and O. Unal, Biologia, 65, 990 (2010).