Copyright (c) 2013 AJC
This work is licensed under a Creative Commons Attribution 4.0 International License.
Study of Chromatographic Column Packing and Its Application
Corresponding Author(s) : Z.Q. Liu
Asian Journal of Chemistry,
Vol. 25 No. 4 (2013): Vol 25 Issue 4
Abstract
This experiment used analyzer and chromatography to make the evaluation of chromatographic packing materials based on silica by means of analysis, separation and purification. Reverse phase silica was prepared by octadecyltrichlorosilane (C18) under normal lab conditions. The column was filled with the prepared silica as chromatographic packing materials and the column efficiency was also evaluated. Then lignans was isolated of from natural extracts using the prepared column. The lignans was purified with high pressure and its purity was also tested with HPLC. In addition the procedure was repeated many times so that the article discussed and tested the performance of chromatographic packing materials based on silica.
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- Q.H. Zhang, L.J. Yan and W.B. Zhang and Y.K. Zhang, Chin. J. Anal. Chem., 36, 572 (2008).
- H.F. Zou, Z. Liu and M.L. Ye, The Capillary Electrochromatography and Application, Science Press, Beijing, p. 30 (2001).
- L.J. Yan, Q.H. Zhang and Y.Q. Feng, W.-B. Zhang, T. Li, L.-H. Zhang and Y.-K. Zhang, J. Chromatogr. A, 1121, 92 (2006).
- L.J. Yan, Q.H. Zhang and W.B. Zhang, Y.Q. Feng, L.H. Zhang, T. Li and Y.K. Zhang, Electrophroesis, 26, 2935 (2005).
- H.L. Luo, J. Sheng and Y.Z. Wan, Mater. Lett., 62, 37 (2008).
- T.J. Yao, Q. Lin, K. Zhang, D.F. Zhao, H. Lv, J.H. Zhang and B. Yang, J. Colloid Interf. Sci., 315, 434 (2007).
References
Q.H. Zhang, L.J. Yan and W.B. Zhang and Y.K. Zhang, Chin. J. Anal. Chem., 36, 572 (2008).
H.F. Zou, Z. Liu and M.L. Ye, The Capillary Electrochromatography and Application, Science Press, Beijing, p. 30 (2001).
L.J. Yan, Q.H. Zhang and Y.Q. Feng, W.-B. Zhang, T. Li, L.-H. Zhang and Y.-K. Zhang, J. Chromatogr. A, 1121, 92 (2006).
L.J. Yan, Q.H. Zhang and W.B. Zhang, Y.Q. Feng, L.H. Zhang, T. Li and Y.K. Zhang, Electrophroesis, 26, 2935 (2005).
H.L. Luo, J. Sheng and Y.Z. Wan, Mater. Lett., 62, 37 (2008).
T.J. Yao, Q. Lin, K. Zhang, D.F. Zhao, H. Lv, J.H. Zhang and B. Yang, J. Colloid Interf. Sci., 315, 434 (2007).