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Vol. 25 No. 3 (2013): Vol 25 Issue 3

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Fractionation of Extract from Ligusticum chuanxiong (Apiaceae) by High Speed Counter Current Chromatography and Their Efficacy in Rice Against S-metolachlor Injury

https://doi.org/10.14233/ajchem.2013.13470
Jing-Bo Li
Department of Pesticide Science, Hunan Agricultural University, Changsha 410128, Hunan, P.R. China
Liang-Wei Du
College of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, Guangxi, P.R. China
Xiao-Mao Zhou
Department of Pesticide Science, Hunan Agricultural University, Changsha 410128, Hunan, P.R. China
Li-Feng Wang
Department of Pesticide Science, Hunan Agricultural University, Changsha 410128, Hunan, P.R. China
Lian-Yang Bai
Department of Pesticide Science, Hunan Agricultural University, Changsha 410128, Hunan, P.R. China ; Hunan Institute of Humanities, Science and Technology, Loudi 417000, Hunan, P.R. China

Corresponding Author(s) : Lian-Yang Bai

bailianyang2005@yahoo.com.cn

Asian Journal of Chemistry, Vol. 25 No. 3 (2013): Vol 25 Issue 3

Abstract

Herbicide S-metolachlor with the high efficacy toward the weed from Cyperaceae and Poaceae is never solely applied in rice field due to its grave inhibition to rice growth. This study was conducted to separate the active compound from Ligusticum chuanxiong Hort, which could protect rice from the injury of S-metolachlor. High speed counter current chromatography was applied to the separation of the crude extract of L. chuanxiong Hort. The crude extract obtained by supercritical fluid extraction was fractionated into four fractions after eluted in the head-to-tail pattern with a n-hexane/ethyl acetate/ethanol/water solvent system (5:4:3:2, v/v/v/v). Two components were successfully separated by high speed counter current chromatography and identified as Z-ligustilide and senkyunolide A by liquid chromatography-mass spectrometry, gas chromatography-mass spectrometry, proton nuclear magnetic resonance (1H NMR) and carbon nuclear magnetic resonance (13C NMR). The corresponding yields with the purity of over 96 % were 40 mg and 15 mg from 500 mg of crude extract in a single run, respectively. In bioassay experiments, results showed that the level of the growth recoveries conferred by Z-ligustilide and senkyunolide A were 100 % and 91 % of untreated control in plant height as well as 94 % and 83 % of untreated control in roots length, respectively. Z-ligustilide has the potential to be a safener with the capability of reversing the effects on rice caused by S-metolachlor.

Keywords

Rice Ligusticum chuanxiong Hort High speed counter current chromatography Z-ligustilide Safener
Li, J.-B., Du, L.-W., Zhou, X.-M., Wang, L.-F., & Bai, L.-Y. (2012). Fractionation of Extract from Ligusticum chuanxiong (Apiaceae) by High Speed Counter Current Chromatography and Their Efficacy in Rice Against S-metolachlor Injury. Asian Journal of Chemistry, 25(3), 1613–1617. https://doi.org/10.14233/ajchem.2013.13470
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