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Study on the Extraction and Purification of Total Flavone in Etirfel Preparation
Corresponding Author(s) : Yunpeng Diao
Asian Journal of Chemistry,
Vol. 25 No. 3 (2013): Vol 25 Issue 3
Abstract
The aim of the study was to optimize and discuss the extraction and purification of total flavone in etirfel preparation using macroporous absorption resin. Orthogonal test was adopted to optimize the purification of total flavone in etirfel preparation, with water as the solvent, using the content of total flavone in etirfel preparation as the evaluation indicator. The extraction temperature, extraction time and solvent consumption were chosen as the investigational factors to screen the optimal resin via the static experiments on the two types of macroporous absorption resin, namely using AB-8 and polyamide to investigate the adsorbtion and elution of total flavone in etirfel preparation by optimizing the extraction process parameters. The optimal extraction condition was obtained using a single 1 h extraction technology, at 50 ºC, with a extract-water ratio of 1:10. The optimal condition for purification of total flavone using 10 g polyamide resin are as follows. The adsorption was carried out at a flow rate of 0.25 mL/min, the loading solution volume was 25 mL, the diameter-height ratio was 1:3 and the eluent flow rate was 1.5 mL/min. The proprietary extraction process for the highest content of total flavone was obtained in this study through the optimization of the extraction and purification, providing a chemical basis for further pharmacological studies.
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References
S. Rukeyamu, Compiler, Uyghur Medicine Pharmaceutics and Compound, Xinjiang People's Medical Publishing House, p. 75 (1987).
Chinese Medical Encyclopedia Uyghur Medicine Volume Office, Chinese Medical Encyclopedia Uyghur Medicine Volume, Xinjiang Technology and Medical Publishing House, p. 10 (1995).
S. Abudukerimu, Xinjiang J. Uyghur Med. College, 3, 35 (1997).
A. Abuduwupuer, Xinjiang J. Uyghur Med. College, 1, 33 (1999).
Maimaitihasimu and S. Yishake, J. Pract. Med., 2, 9 (2000).
A. Sidike and Bahaerguli, J. Uygur Med., 2, 18 (2002).
Y. Kadier and A. Keyimu, J. Uygur Med., 4, 19 (2002).
H.G. Maimaiti, Xinjiang J. Uyghur Med. College, 3, 26 (1997).
N. Aishan and P. Abula, Xinjiang J. Uyghur Med. College, 2, 43 (1996).
A.J. Sidike and A. Abudureyimu, Xinjiang J. Uyghur Med. College, 1, 24 (2001).
M.J. Abulizi, J. Uygur Med., 1, 10 (2002).
M. Basiti, Xinjiang J. Uyghur Med. College, 2, 47 (1997).
K. Tuerdi and A. Wumaieraji, Xinjiang J. Uyghur Med. College, 2, 32 (2001).
A. Abudurexiti and K. Shayidi, Xinjiang J. Uyghur Med. College, 3, 22 (2000).
A. Aximu, J. Uygur Med., 1, 30 (1991).
A. Maimaiti, J.G. Liu and M. Kamili, J. Med. Pharm. Chin. Minorities, 9, 60 (2008).
E.M. Silva, D.R. Pompeu, Y. Larondelle and H. Rogez, Sep. Purif. Technol., 53, 274 (2007).
J. Lei, Strait Pharm. J., 20, 66 (2008).
Y. Cun and Y. Sha, Zhejiang J. Preventive Med., 14, F003 (2002).
L.J. He, H. Chen, X. Lin and S.Z. Jiang, Strait Pharm. J., 22, 61 (2010).
L.Z. Lin, H.F. Zhao, Y. Dong, B. Yang and M.M. Zhao, Food Chem., 69, 1016 (2011).
Z.Y. Zhao, L.L. Dong, Y.L. Wu and F. Lin, Food Bioprod. Processing, 205, 1 (2010).
L. Tang, Y. Ma, C.Q. Cai, J.S. Li, Y. Liu, B. Wang and X. Yao, Acta China Med. Pharmacol., 39, 51 (2011).
J. Lin, C.L. Li, L.Q. Ruan and Z.L. Lin, J. Shanxi Med. Univ., 41, 876 (2010).
Q.L. Tan, D. Liu, Y.B. Li and D.Y. Peng, J. Anhui Tradit. Chin. Med. College, 3, 62 (2009)