Simultaneous Estimation of Atorvastatin Calcium and Fenofibrate in Rabbit Plasma by RP-HPLC

Somnath D. Bhinge; Sharangauda M. Malipatil; Abhijeet Jondhale; Anil S. Savali

doi:10.14233/ajchem.2013.13310

Issue

Vol. 25 No. 2 (2013): Vol 25 Issue 2

Issue Published : September 6, 2012

This work is licensed under a Creative Commons Attribution 4.0 International License.

Simultaneous Estimation of Atorvastatin Calcium and Fenofibrate in Rabbit Plasma by RP-HPLC

https://doi.org/10.14233/ajchem.2013.13310

Somnath D. Bhinge

Rajarambapu College of Pharmacy, Rajaram Nagar, Kasegaon-415 404, India

Sharangauda M. Malipatil

HKES’s College of Pharmacy, Gulbarga-585 105, India

Abhijeet Jondhale

RMES’s College of Pharmacy, Balaji Nagar, Old Jewargi Road, Gulbarga-585 102, India

Anil S. Savali

RMES’s College of Pharmacy, Balaji Nagar, Old Jewargi Road, Gulbarga-585 102, India

Corresponding Author(s) : Somnath D. Bhinge

somu1245@yahoo.co.in

Asian Journal of Chemistry, Vol. 25 No. 2 (2013): Vol 25 Issue 2
Article Published : September 8, 2012

Abstract

An accurate, rapid and simple reversed-phase high performance liquid chromatography (RP-HPLC) method was developed and validated for simultaneous estimation of atorvastatin calcium and fenofibrate in rabbit plasma. Plasma samples were treated with acetonitrile to precipitate proteins. Chromatographic separation was accomplished using CAPCELL PAK C₈ (4.6 mm × 250 mm, 5 mm) analytical column with a mobile phase consisting of phosphate buffer and acetonitrile (28:72). Detection and quantification were performed by UV/VIS detection at 260 nm. The lower limits of detection and quantification were 0.05 μg mL^-1 and 0.20 μg mL^-1 for atorvastatin calcium and 0.07 μg mL^-1 and 0.35 μg mL^-1 for fenofibrate respectively. The calibration curves are linear over the concentration range 1 to 40 μg mL^-1 for both atorvastatin and fenofibrate in rabbit plasma. The method was quantitatively evaluated in terms of linearity, precision, accuracy, recovery, selectivity and stability. The proposed method is simple, convenient and suitable for the analysis of atorvastatin calcium and fenofibrate in rabbit plasma.

Keywords

Atorvastatin calcium Fenofibrate Rabbit plasma High performance liquid chromatography

D. Bhinge, S., M. Malipatil, S., Jondhale, A., & S. Savali, A. (2012). Simultaneous Estimation of Atorvastatin Calcium and Fenofibrate in Rabbit Plasma by RP-HPLC. Asian Journal of Chemistry, 25(2), 981–985. https://doi.org/10.14233/ajchem.2013.13310

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Y. Shah, Z. Iqbal, L. Ahmed, A. Khan, M.D.I. Khan, S. Nazir and F. Nasir, J. Chromatogr. B, 879, 557 (2011).

A. Sassano and L.C. Platanias, Cancer Lett., 260, 11 (2008).

R.P.M. Wong and T.M.E. Davis, Antmicrob. Agents Chem., 53, 2212 (2009).

A.V. Ferreira, G.G. Parreira, A. Green and L.M. Botion, Metabolism, 55, 731 (2006).

D. Guillen, F. Cofan, E. Ros, O. Millan, M. Cofen, A. Rimola and M. Brunet, Anal. Bioanal. Chem., 394, 1687 (2009).

M. Hermann, H. Christensen and J. Reubsaet, Anal. Bioanal. Chem., 382, 1242 (2005).

W.W. Bullen, R.A. Miller and R.N. Hayes, J. Am. Soc. Mass Spectrom., 10, 55 (1999).

G. Bahrami, B. Mohammadi and S. Mirzaeei, J. Chromatogr. B, 826, 41 (2005).

A. Zarghi, A. Shafaati, S.M. Foroutan and A.A. Khoddam, Arzneimittel Forchung, 55, 451 (2005).

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R. Petkovska, C. Cornett and A. Dimitrovska, Anal. Lett., 41, 992 (2008).

A. Mohammadi, N. Rezanour, M.A. Dogaheh, F.G. Bidhorbed, M. Hashem and R. Walker, J. Chromatogr. B, 846, 215 (2007).

V. Patel, R. Baldha and D. Patel, Asian J. Chem., 22, 2511 (2010).

K.R. Gupta, S.S. Askarkar and S.G. Wadodkar, Eur. J. Anal. Chem., 4, 294 (2009).

H. Freddy and V. Chaudhari, Asian J. Chem., 17, 2502 (2005).

X.S. Miao and C.D. Metcalfe, J. Chromatogr. A, 998, 133 (2003).

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N. Jain, R. Raghuvanshi and D. Jain, Indian J. Pharm. Sci., 70, 263 (2008).

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Quality Assurance of Pharmaceuticals, A Compendium of Guideline and Related Materials, India, Universal Publishing, vol. 1 (1992).

L.R. Snyder, J.J. Kirkland and J.L. Glajch, Practical HPLC Method Development, John Wiley & Sons, USA (1997).

M.M. Bhatti, G.D. Hanson and L. Schultz, J. Pharm. Biomed. Anal., 16, 1233 (1998).

D. Chollet, E. Castella, P. Combe and V. Arnera, J. Chromatogr. B: Biomed. Appl., 683, 237 (1996).

K.M. Patil and S.L. Bodhankar, J. Pharm. Biomed. Anal., 39, 181 (2005).

R.T. Sane, S. Menon, A.Y. Deshpande and A. Jain, Chromatographia, 61, 137 (2005)

Issue

Vol. 25 No. 2 (2013): Vol 25 Issue 2

Simultaneous Estimation of Atorvastatin Calcium and Fenofibrate in Rabbit Plasma by RP-HPLC

Corresponding Author(s) : Somnath D. Bhinge

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