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Isolation and Quantification of 14-Deoxy-11,12-didehydroandrographolide in Andrographis paniculata by HPLC
Corresponding Author(s) : S. Narasimhan
Asian Journal of Chemistry,
Vol. 25 No. 2 (2013): Vol 25 Issue 2
Abstract
Present work focuses on the development and standardization of a chromatographic method for quantification of 14-deoxy-11,12-didehydroandrographolide (DDAP), from Andrographis paniculata by HPLC method. The structure of isolated marker was characterized and confirmed by various advanced spectroscopic methods. A simple, precise, accurate and rapid high pressure liquid chromatographic method has been developed and validated for the estimation of 14-deoxy-11,12-didehydroandrographolide in different extract of A. paniculata. Chromatographic separation was carried out on Phenomenex Gemini RP C-18 (5 μ, 250 × 4.6 mm) at room temperature using a (isocratic phase) mobile phase consisting of flow rate of 0.5 mL/min, with UV detection at 235 nm. The method has been studied for linearity, precision, accuracy, limit of detection (LOD) and limit of quantification (LOQ). The proposed HPLC method was simple, accurate, precise and suitable for performing the quality control of crude extract and herbal formulation.
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- D. Bensky and A.Gamble, Chinese Herbal Medicine Materia Medica, Revised Edition; Eastland Press: Seattle, WA, USA, p. 9 (1993).
- R.S. Chang, L. Ding, G.Q. Chen, Q.C. Pan, Z.L. Zhao and K.M. Smith, Proc. Soc. Exp. Biol. Med., 197, 59 (1991).
- C. Calabrese, S.H. Berman, J.G. Babish, X. Ma, L. Shinto, M. Dorr, K. Wells, C.A. Wenner and L.J. Standish. Phytother. Res., 14, 333 (2000).
- Y.C. Shen, C.F. Chen and W.F. Chiou, Br. J. Pharmacol., 135, 399 (2002).
- P. Misra, N.L. Pal, P.Y. Guru, J.C. Katiyar, V. Srivastava and J.S. Tandon, Int. J. Pharmacog., 30, 263 (1992).
- A. Puri, R. Saxena, R.P. Saxena, K.C. Saxena, V. Srivastava and J.S. Tandon, J. Nat. Prod., 56, 995 (1993).
- D. See, S. Mason and R. Roshan, Immunol. Invest., 31, 137 (2002).
- A. Kapil, I.B. Koul, S.K. Banerjee and B.D. Gupta, Biochem. Pharmacol., 46, 182 (1993).
- H.Y. Zhao and W.Y. Fang, Chin. Med. J., 104, 770 (1991).
- T. Matsuda, M. Kuroyanagi, S. Sugiyama, K. Umehara, A. Ueno and K. Nishi, Chem. Pharm. Bull. (Tokyo), 42, 1216 (1994).
- X.F. Zhang and B.K. Tan, Clin. Exp. Pharmacol. Physiol., 27, 358 (2000).
- C.Y. Zhang and B.K. Tan, Clin. Exp. Pharmacol. Physiol., 23, 675 (1996).
- Y.C. Shen, C.F. Chen and W.F. Chiou, Planta Med., 66, 314 (2000).
- R. Chander, V. Srivastava, J.S. Tandon and N.K. Kapoor, Int. J. Pharmacog., 33, 135 (1995).
- C. Zhang, M. Kuroyangi and B.K. Tan, Pharmacol. Res., 38, 413 (1998).
- P. Misra, N.L. Pal, P.Y. Guru, J.C. Katiyar, V. Srivastava and J.S. Tandon, Int. J. Pharmacog., 30, 263 (1992).
- K.N. Gaind, R.N. Dai and R.N. Kaul, Indian J. Pharm., 25, 255 (1963).
- D. Shanbhag and S. Jayaraman, Asian J. Chem., 20, 509 (2008).
- P.Y. Zhu, N.B. Peng and W.J. Jiang, Chin. J. Pharm. Anal., 4, 34 (1984).
- A. Sharma, K. Lal and S.S. Handa, Phytochem. Anal., 3, 129 (1992).
- S. Sudhanshu, C.J. Dharam, M.G. Madan, S.B. Rajendra, O.M. Hari and P.S. Ram, Phytochem. Anal., 11, 34 (2000).
- ICH topic Q2B, Validation of Analytical Procedures, Validation of Analytical Procedures: Methodology, Note for Guidance on Validation and Analytical Procedures: Methodology (1996).
References
D. Bensky and A.Gamble, Chinese Herbal Medicine Materia Medica, Revised Edition; Eastland Press: Seattle, WA, USA, p. 9 (1993).
R.S. Chang, L. Ding, G.Q. Chen, Q.C. Pan, Z.L. Zhao and K.M. Smith, Proc. Soc. Exp. Biol. Med., 197, 59 (1991).
C. Calabrese, S.H. Berman, J.G. Babish, X. Ma, L. Shinto, M. Dorr, K. Wells, C.A. Wenner and L.J. Standish. Phytother. Res., 14, 333 (2000).
Y.C. Shen, C.F. Chen and W.F. Chiou, Br. J. Pharmacol., 135, 399 (2002).
P. Misra, N.L. Pal, P.Y. Guru, J.C. Katiyar, V. Srivastava and J.S. Tandon, Int. J. Pharmacog., 30, 263 (1992).
A. Puri, R. Saxena, R.P. Saxena, K.C. Saxena, V. Srivastava and J.S. Tandon, J. Nat. Prod., 56, 995 (1993).
D. See, S. Mason and R. Roshan, Immunol. Invest., 31, 137 (2002).
A. Kapil, I.B. Koul, S.K. Banerjee and B.D. Gupta, Biochem. Pharmacol., 46, 182 (1993).
H.Y. Zhao and W.Y. Fang, Chin. Med. J., 104, 770 (1991).
T. Matsuda, M. Kuroyanagi, S. Sugiyama, K. Umehara, A. Ueno and K. Nishi, Chem. Pharm. Bull. (Tokyo), 42, 1216 (1994).
X.F. Zhang and B.K. Tan, Clin. Exp. Pharmacol. Physiol., 27, 358 (2000).
C.Y. Zhang and B.K. Tan, Clin. Exp. Pharmacol. Physiol., 23, 675 (1996).
Y.C. Shen, C.F. Chen and W.F. Chiou, Planta Med., 66, 314 (2000).
R. Chander, V. Srivastava, J.S. Tandon and N.K. Kapoor, Int. J. Pharmacog., 33, 135 (1995).
C. Zhang, M. Kuroyangi and B.K. Tan, Pharmacol. Res., 38, 413 (1998).
P. Misra, N.L. Pal, P.Y. Guru, J.C. Katiyar, V. Srivastava and J.S. Tandon, Int. J. Pharmacog., 30, 263 (1992).
K.N. Gaind, R.N. Dai and R.N. Kaul, Indian J. Pharm., 25, 255 (1963).
D. Shanbhag and S. Jayaraman, Asian J. Chem., 20, 509 (2008).
P.Y. Zhu, N.B. Peng and W.J. Jiang, Chin. J. Pharm. Anal., 4, 34 (1984).
A. Sharma, K. Lal and S.S. Handa, Phytochem. Anal., 3, 129 (1992).
S. Sudhanshu, C.J. Dharam, M.G. Madan, S.B. Rajendra, O.M. Hari and P.S. Ram, Phytochem. Anal., 11, 34 (2000).
ICH topic Q2B, Validation of Analytical Procedures, Validation of Analytical Procedures: Methodology, Note for Guidance on Validation and Analytical Procedures: Methodology (1996).