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Studies on the Reaction of Allyl Glycidyl Ether with Gelatin by Van Slyke Method
Corresponding Author(s) : T.D. Li
Asian Journal of Chemistry,
Vol. 25 No. 2 (2013): Vol 25 Issue 2
Abstract
Gelatin was grafted by allyl glycidyl ether (AGE) at 40 ºC under solution pH ranges from 8-13. The Van Slyke method, used for the amino acids analyses quantitatively, was used to determine the conversion rate of the free -NH2 groups of gelatin. The results showed that the grafting of epoxy compound onto gelatin mainly took place on free -NH2 groups of gelatin. The highest conversion rate of the free -NH2 groups was 67.72 %, while the solution pH was 11. The results were consistent with those obtained by colorimetry and gravimetric analysis methods. By comparing with the results obtained from the three analysis methods, we found that the Van Slyke method was the most convenient and reliable method to measure the conversion rate of the free -NH2 groups. The Van Slyke method is a novel and good method to measure the conversion rate of the free -NH2 groups.
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- S.C.G. Leeuwenburgh, J. Jo, H. Wang, M. Yamamoto, J.A. Jansen and Y. Tabata, Biomacromolecules, 11, 2653 (2010).
- J.J. Han, P. Lazarovici, C. Pomerantz, X.S. Chen, Y. Wei and P.I. Lelkes, Biomacromolecules, 12, 399 (2011).
- A. Balaji, V.P. Pandey, M.S. Srinath and R. Manavalan, Asian J. Chem., 21, 6277 (2009).
- S. Jha, S.N. Dikshit and G. Pandey, Asian J. Chem., 23, 4098 (2011).
- Y.H. Cheng, S.H. Yang and F.H. Lin, Biomaterials, 32, 6953 (2011).
- L. Kurniawan, G.G. Qiao and X.P. Zhang, Biomacromolecules, 8, 2909 (2007).
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- R. Zeeman, P.J. Dijkstra, P.B.Van Wachem, M.J. Van Luyn, M. Hendriks, P.T. Cahalan and J. Feijen, Biomaterials, 20, 921 (1998).
- R. Zeeman, P.J. Dijkstra, P.B. Van Wachem, M.J. Van Luyn, M. Hendriks, P.T. Cahalan and J. Feijen, J. Biomed. Mater. Res., 51, 541 (2000).
- H.W. Sung and J.S. Shih, J. Appl. Biomater. Biomech., 6, 185 (1995).
- Y. Di, R.J. Heath and A. Long, J. Soc. Leath. Technol. Ch., 90, 93 (2006).
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- R. Wilson, Anal. Lett., 24, 1983 (1991).
- D.D. van Slyke, J. Biol. Chem., 83, 425 (1929).
- C. Fraenkel and L. Heinz, J. Biol. Chem., 148, 453 (1943).
- T.D. Li, X.L. Tang, Q.W. Jiang and J. Xu, Chinese Patent 2011-10249480.1 (2011).
- T.D. Li, X.L. Tang, Q.W. Jiang and J. Xu, Chinese Patent 201120316998.8 (2011).
- H.W. Sung, C.S. Hsu and Y.S. Lee, J. Biomed. Mater. Res., 31, 511 (1996).
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References
S.C.G. Leeuwenburgh, J. Jo, H. Wang, M. Yamamoto, J.A. Jansen and Y. Tabata, Biomacromolecules, 11, 2653 (2010).
J.J. Han, P. Lazarovici, C. Pomerantz, X.S. Chen, Y. Wei and P.I. Lelkes, Biomacromolecules, 12, 399 (2011).
A. Balaji, V.P. Pandey, M.S. Srinath and R. Manavalan, Asian J. Chem., 21, 6277 (2009).
S. Jha, S.N. Dikshit and G. Pandey, Asian J. Chem., 23, 4098 (2011).
Y.H. Cheng, S.H. Yang and F.H. Lin, Biomaterials, 32, 6953 (2011).
L. Kurniawan, G.G. Qiao and X.P. Zhang, Biomacromolecules, 8, 2909 (2007).
G. Vargas, J.L. Acevedo and J. López, Mater. Lett., 62, 3656 (2008).
R. Zeeman, P.J. Dijkstra, P.B.Van Wachem, M.J. Van Luyn, M. Hendriks, P.T. Cahalan and J. Feijen, Biomaterials, 20, 921 (1998).
R. Zeeman, P.J. Dijkstra, P.B. Van Wachem, M.J. Van Luyn, M. Hendriks, P.T. Cahalan and J. Feijen, J. Biomed. Mater. Res., 51, 541 (2000).
H.W. Sung and J.S. Shih, J. Appl. Biomater. Biomech., 6, 185 (1995).
Y. Di, R.J. Heath and A. Long, J. Soc. Leath. Technol. Ch., 90, 93 (2006).
A. Gantar, A. Šebenik and M. Kavcic, Polymer, 28, 1403 (1987).
H. Saito, T. Taguchi and H. Kobayashi, Mater. Sci. Eng. C, 24, 781 (2004).
R. Wilson, Anal. Lett., 24, 1983 (1991).
D.D. van Slyke, J. Biol. Chem., 83, 425 (1929).
C. Fraenkel and L. Heinz, J. Biol. Chem., 148, 453 (1943).
T.D. Li, X.L. Tang, Q.W. Jiang and J. Xu, Chinese Patent 2011-10249480.1 (2011).
T.D. Li, X.L. Tang, Q.W. Jiang and J. Xu, Chinese Patent 201120316998.8 (2011).
H.W. Sung, C.S. Hsu and Y.S. Lee, J. Biomed. Mater. Res., 31, 511 (1996).
R. Zeeman, P.J. Dijkstra and P.B. Wachem, Biomed. Mater. Res., 46, 424 (1999).
H.S.M. Zahangir, M. Ohnishi, S. Kanoh and E. Niwa, Fisheries Sci., 2, 327 (1999).