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Vol. 25 No. 2 (2013): Vol 25 Issue 2

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Protein Immobilization on A Biotinylated Polymer Coating

https://doi.org/10.14233/ajchem.2013.12921
Jingzhe Zhang
Department of Orthopedics, China-Japan Union Hospital, Jilin University, Changchun 130033, P.R. China
Xintong Wang
Bethune Medical School, Jilin University, Changchun 130021, P.R. China
Limei Liu
Jilin Province Power Hospital, Changchun 130022, P.R. China
Peimin Chen
Jilin Province Power Hospital, Changchun 130022, P.R. China
Xiaohong Zhang
School of Pharmaceutical Sciences, Jilin University, Changchun 130021, P.R. China
Tiancheng Lu
College of Life Sciences, Jilin Agricultural University, Changchun 130118, P.R. China

Corresponding Author(s) : Xiaohong Zhang

xiaohongzhang88@sina.com

Asian Journal of Chemistry, Vol. 25 No. 2 (2013): Vol 25 Issue 2

Abstract

A polymer coating to specifically immobilize proteins was constructed from a biodegradable amphiphilic triblock copolymer, biotinylated poly(ethylene glycol)-b-poly(L-lactide)-b-poly(Llysine) (PEG-PLA-PLL/biotin), accompanied by covering it with a layer of blocking agent consisting of gelatin, poly(N-vinyl pyrrolidone) and casein. The bioactive surface was characterized by a confocal laser scanning microscope analysis by means of consecutive immobilization of non-labeled streptavidin, biotinylated rabbit antigoat monoclonal antibodies and FITC-labeled goat globulin. In results, the bioactive surface repelled nonspecific protein and allowed specific biotin-streptavidin interaction; meanwhile the immobilized streptavidin kept its bioactivity. In addition, the PEG-PLA-PLL/biotin coating could enhance the attachment and proliferation of mouse L929 fibroblasts, suggesting that it had excellent biocompatibility. The PEG-PLA-PLL/biotin is potential to become a bioactive material for protein immobilization and biodegradable scaffolds.

Keywords

Poly(L-lactide) Biotin Protein immobilization Bicompatibility
Zhang, J., Wang, X., Liu, L., Chen, P., Zhang, X., & Lu, T. (2012). Protein Immobilization on A Biotinylated Polymer Coating. Asian Journal of Chemistry, 25(2), 811–816. https://doi.org/10.14233/ajchem.2013.12921
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