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Tbfmoc Methioine: Its Synthesis and Application for the Solid Phase Synthesis and Purification of Ubiquitin C-Hydrazide
Corresponding Author(s) : P. Wang
Asian Journal of Chemistry,
Vol. 25 No. 2 (2013): Vol 25 Issue 2
Abstract
The new reagent Tbfmoc-Met-OH is synthesized and used as a building block to replace Fmoc-Met-OH for the solid phase synthesis of ubiquitin C-hydrazide, an unnatural protein containing 76 amino acids and bring the Tbfmoc group into the synthetic product efficiently. The attached Tbfmoc group can provide good help for the product separation and improve the purification efficiency significantly.
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References
D. Mukhopadhyay and H. Riezman, Science, 315, 201 (2007).
M. Hochstrasser, Nature, 458, 422 (2009).
V.G. Bhoj and Z.J. Chen, Nature, 458, 430 (2009).
S. Bergink and S. Jentsch, Nature, 458, 461 (2009).
S. Virdee, Y. Ye, D.P. Nguyen, D. Komander and J.W. Chin, Nat. Chem. Biol., 6, 750 (2010).
N.X. Zhang, Q.H. Wang, A. Ehlinger, L. Randles, J.W. Lary, Y. Kang, A. Haririnia, A.J. Storaska, J.L. Cole, D. Fushman and K.J. Walters, Mol. Cell, 35, 280 (2009).
A.R. Brown, S.L. Irving and R. Ramage, Tetrahedron Lett., 34, 7129 (1993).
A.R. Brown, S.L. Irving, R. Ramage and G. Raphy, Tetrahedron, 51, 11815 (1995).
N. Robertson and R. Ramage, J. Chem. Soc. Perkins Trans I, 8, 1015 (1999).
L.A. Carpino and G.Y. Han, J. Am. Chem. Soc., 92, 5748 (1970).