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Vol. 25 No. 1 (2013): Vol 25 Issue 1

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Investigation on Interaction of Buflomedil Hydrochloride with Trypsin: A Spectroscopic Analysis

https://doi.org/10.14233/ajchem.2013.13028
Xiaoling Li
Department of Chemistry and Materials Science, Huaibei Normal University, Huaibei 235000, Anhui Province, P.R. China ; Department of Chemistry and Life Science, Suzhou University, Suzhou 234000,Anhui Province, P.R. China
Xiaoping Zhang
Department of Chemistry and Materials Science, Huaibei Normal University, Huaibei 235000, Anhui Province, P.R. China
Li Wang
Department of Chemistry and Materials Science, Huaibei Normal University, Huaibei 235000, Anhui Province, P.R. China
Xiaoyan Gao
Department of Chemistry and Materials Science, Huaibei Normal University, Huaibei 235000, Anhui Province, P.R. China
Wujie Zhao
Department of Chemistry and Materials Science, Huaibei Normal University, Huaibei 235000, Anhui Province, P.R. China
Yanqin Zi
Department of Chemistry and Materials Science, Huaibei Normal University, Huaibei 235000, Anhui Province, P.R. China

Corresponding Author(s) : Yanqin Zi

szxylxl@163.com

Asian Journal of Chemistry, Vol. 25 No. 1 (2013): Vol 25 Issue 1

Abstract

The interaction between buflomedil hydrochloride and trypsin has been studied through fluorescence spectroscopy and UV/VIS spectrophotometry. With the addition of buflomedil hydrochloride, the fluorescence emission intensity of trypsin was quenched. The number of binding sites and the binding constants were measured by the fluorescence quenching method. Thermodynamic parameters DH, DG and DS at three temperatures (293 K, 300 K, 310 K) were calculated. The force acting between buflomedil hydrochloride and trypsin was mainly a hydrophobic interaction. The binding distance between the donor (trypsin) and acceptor (buflomedil hydrochloride) was 0.862 nm according to Förster's non-radiational energy transfer theory.

Keywords

Buflomedil hydrochloride Trypsin Fluorescence quenching Stern-volmer equation Förster's resonance energy transfer theory
Li, X., Zhang, X., Wang, L., Gao, X., Zhao, W., & Zi, Y. (2012). Investigation on Interaction of Buflomedil Hydrochloride with Trypsin: A Spectroscopic Analysis. Asian Journal of Chemistry, 25(1), 327–330. https://doi.org/10.14233/ajchem.2013.13028
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