Copyright (c) 2013 AJC
This work is licensed under a Creative Commons Attribution 4.0 International License.
Taxonomy and Characterization of Bioactive Metabolites from a New Marine Microorganism Strain MLA-21
Corresponding Author(s) : Tao Xi
Asian Journal of Chemistry,
Vol. 25 No. 1 (2013): Vol 25 Issue 1
Abstract
Marine actinomyces MLA-21 was isolated from Arctic sediment samples. The strain was identified by the methods of morphology, physiological and chemotaxonomic characterization and 16S rRNA gene sequence analysis. The results showed that the strain was a new marine variable species of S. coelicolour. Two active compounds were isolated and purified from the fermentation of the strain and their antitumor activity against HepG-2, MCF-7 and A549 cell lines were defined by vitro cytotoxic activity assay. Their structures were elucidated as methyl p-hydroxybenzoate (1) and 3-(4-propylphenoxy)-propanoic acid (2) by 1H and 13C NMR and EI-MS spectroscopic methods. Compounds 1 and 2 were isolated from marine microorganism for the first time.
Keywords
Download Citation
Endnote/Zotero/Mendeley (RIS)BibTeX
- S.T. Williams, M. Goodfellow, G. Alderson, E.M.H. Wellington, P.H.A. Sneath and M.J. Sackin, Gen. Microbiol, 129, 1747 (1983).
- S. Miyadoh, Actinomycetologica, 9, 100 (1993).
- J. Berdy, J. Antibiot, 58, 1 (2005).
- J.R. Zgoda and J.R. Porter, Pharmaceut. Biol., 39, 221 (2001).
- K. Yukimura, R. Nakai, S. Kohshima, J. Uetake, H. Kanda and T. Naganuma, Polar Science, 3, 163 (2009).
- S. D'Amico, T. Collins, J.C. Marx, G. Feller, C. Gerday and C. Gerday, EMBO reports, 7, 385 (2006).
- P. Ellaiah, D. Kalyan, V.S. Rao and B.V. Rao, Hindustan Antibiot. Bull., 38, 48 (1996).
- F.A. Rainey, N.W. Rainey, R.M. Kroppenstedt, E. Stackebrandt, Int. J. Syst. Bacteriol., 46, 1088 (1996).
- W.G. Weisburg, S.M. Barns, D.A. Pelletier and D.J. Lane, J. Bacteriol., 173, 697 (1991).
- J. Brosius, M.L. Palmer, P.J. Kennedy and H.F. Noller, Proc. Nat. Acad Sci., 75, 4801 (1978).
- J.D. Thompson, T.J. Gibson, F. Plewniak, F. Jeanmougin and D.G. Higgins, Nucleic Acids Res., 25, 4876 (1997).
- N. Saitou and M. Nei, Mol. Biol. Evol., 4, 406 (1987).
- S. Kumar, K. Tamura, I.B. Jakobsen, M. Nei, MEGA 2.1: Molecular Evolutionary Genetics Analysis Software (2001).
- E.B. Shirling and D. Gottlieb, Int. J. Syst. Bacteriol., 16, 313 (1966).
- S.A. Waksman, The Actinomycetes, The Ronald Press Company, New York (1967).
- A.J. Holding, Methods Microbiol., 6, 1 (1971).
- Y.M. Seo and S.W. Hong, Korean J. Microbiol., 15, 93 (1977).
- M.P. Lechevalier and H.A. Lechevalier, Int. J. Syst. Bacteriol., 20, 435 (1970).
- J.L. Staneck and G.D. Roberts, Appl. Microbiol., 28, 226 (1974).
- T. Mosmann, Immunol Methods, 65, 55 (1983).
- S.J. Higginbotham and C.D. Murphy, Microbiol. Res., 165, 82 (2010).
- F.C. Domingues, J.A. Queiroza and J.M.S. Cabralb, Enzyme and Microb. Technol., 26, 394 (2000).
- M.D. Awouafack, S.F. Kouam, H. Hussain, D. Ngamga, P. Tane, B. Schulz, I.R. Green and K. Krohn, Planta Med., 74, 50 (2008).
- A. Macone, E. Lendaro, A. Comandini, I. Rovardi, R.M. Matarese A. Carraturo and A. Bonamore, Bioorg. Med. Chem., 17, 6003 (2009).
- A. Kavitha, P. Prabhakar, M. Vijayalakshmi and Y. Venkateswarlu, Res. Microbiol., 161, 335 (2010).
References
S.T. Williams, M. Goodfellow, G. Alderson, E.M.H. Wellington, P.H.A. Sneath and M.J. Sackin, Gen. Microbiol, 129, 1747 (1983).
S. Miyadoh, Actinomycetologica, 9, 100 (1993).
J. Berdy, J. Antibiot, 58, 1 (2005).
J.R. Zgoda and J.R. Porter, Pharmaceut. Biol., 39, 221 (2001).
K. Yukimura, R. Nakai, S. Kohshima, J. Uetake, H. Kanda and T. Naganuma, Polar Science, 3, 163 (2009).
S. D'Amico, T. Collins, J.C. Marx, G. Feller, C. Gerday and C. Gerday, EMBO reports, 7, 385 (2006).
P. Ellaiah, D. Kalyan, V.S. Rao and B.V. Rao, Hindustan Antibiot. Bull., 38, 48 (1996).
F.A. Rainey, N.W. Rainey, R.M. Kroppenstedt, E. Stackebrandt, Int. J. Syst. Bacteriol., 46, 1088 (1996).
W.G. Weisburg, S.M. Barns, D.A. Pelletier and D.J. Lane, J. Bacteriol., 173, 697 (1991).
J. Brosius, M.L. Palmer, P.J. Kennedy and H.F. Noller, Proc. Nat. Acad Sci., 75, 4801 (1978).
J.D. Thompson, T.J. Gibson, F. Plewniak, F. Jeanmougin and D.G. Higgins, Nucleic Acids Res., 25, 4876 (1997).
N. Saitou and M. Nei, Mol. Biol. Evol., 4, 406 (1987).
S. Kumar, K. Tamura, I.B. Jakobsen, M. Nei, MEGA 2.1: Molecular Evolutionary Genetics Analysis Software (2001).
E.B. Shirling and D. Gottlieb, Int. J. Syst. Bacteriol., 16, 313 (1966).
S.A. Waksman, The Actinomycetes, The Ronald Press Company, New York (1967).
A.J. Holding, Methods Microbiol., 6, 1 (1971).
Y.M. Seo and S.W. Hong, Korean J. Microbiol., 15, 93 (1977).
M.P. Lechevalier and H.A. Lechevalier, Int. J. Syst. Bacteriol., 20, 435 (1970).
J.L. Staneck and G.D. Roberts, Appl. Microbiol., 28, 226 (1974).
T. Mosmann, Immunol Methods, 65, 55 (1983).
S.J. Higginbotham and C.D. Murphy, Microbiol. Res., 165, 82 (2010).
F.C. Domingues, J.A. Queiroza and J.M.S. Cabralb, Enzyme and Microb. Technol., 26, 394 (2000).
M.D. Awouafack, S.F. Kouam, H. Hussain, D. Ngamga, P. Tane, B. Schulz, I.R. Green and K. Krohn, Planta Med., 74, 50 (2008).
A. Macone, E. Lendaro, A. Comandini, I. Rovardi, R.M. Matarese A. Carraturo and A. Bonamore, Bioorg. Med. Chem., 17, 6003 (2009).
A. Kavitha, P. Prabhakar, M. Vijayalakshmi and Y. Venkateswarlu, Res. Microbiol., 161, 335 (2010).