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Vol. 25 No. 1 (2013): Vol 25 Issue 1

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Determination of Human Serum Albumin with Tetra-amino Copper Phthalocyanine by Resonance Light Scattering Technique

https://doi.org/10.14233/ajchem.2013.13427
Zhongjin Xue
Key Laboratory of Instrumentation Science and Dynamic, Measurement of Ministry of Education, North University of China, Taiyuan 030051, Shanxi Province, P.R. China
Xuejun Zhang
Department of Chemistry, College of Science, North University of China, Taiyuan 030051, Shanxi Province, P.R. China
Gao Wang
National Key Laboratory of Science and Technology on Electronic Test and Measurement, North University of China, Taiyuan 030051, Shanxi Province, P.R. China
Dan Zhang
Department of Chemistry, College of Science, North University of China, Taiyuan 030051, Shanxi Province, P.R. China
Hanchang Zhou
Key Laboratory of Instrumentation Science and Dynamic, Measurement of Ministry of Education, North University of China, Taiyuan 030051, Shanxi Province, P.R. China

Corresponding Author(s) : Xuejun Zhang

lzhx@nuc.edu.cn

Asian Journal of Chemistry, Vol. 25 No. 1 (2013): Vol 25 Issue 1

Abstract

Resonance light scattering has been extensively used in the detection of concentrations of biological macromolecules. For the development of resonance light scattering based protein detection sensor at the point-of-care, it is necessary to make the enhanced resonance light scattering wavelength move to long wave direction to improve the detection sensitivity. A method for determination of protein was developed in presence of tetra-amino copper phthalocyanine (TACuPc) by resonance light scattering technique. In acidic solution, the interaction between TACuPc and human serum albumin yielded strongly enhanced resonance light scattering signals at wavelength 454 nm and the enhanced intensity of resonance light scattering was proportional to the concentration of proteins. The effects of pH and interfering species on the determination of protein were examined. Under optimal conditions, the linear range of human serum albumin is 0-2 μg mL-1 and the detection limit is 19.37 ng mL-1.

Keywords

Protein Human serum albumin Resonance light scattering Tetra-amino copper phthalocyanine
Xue, Z., Zhang, X., Wang, G., Zhang, D., & Zhou, H. (2012). Determination of Human Serum Albumin with Tetra-amino Copper Phthalocyanine by Resonance Light Scattering Technique. Asian Journal of Chemistry, 25(1), 529–532. https://doi.org/10.14233/ajchem.2013.13427
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