1Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Education, Nanjing 210095, P.R. China
2Jinling Institute of Technology, Nanjing 211169, P.R. China
3Shanghai Institute of Applied Physcis, Chinese Academy of Sciences, Shanghai 201800, P.R. China
*Corresponding author: Email: gaoxw@njau.edu.cn
Huijun Wu1
1Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Education, Nanjing 210095, P.R. China
2Jinling Institute of Technology, Nanjing 211169, P.R. China
3Shanghai Institute of Applied Physcis, Chinese Academy of Sciences, Shanghai 201800, P.R. China
*Corresponding author: Email: gaoxw@njau.edu.cn
Yang Yang1
1Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Education, Nanjing 210095, P.R. China
2Jinling Institute of Technology, Nanjing 211169, P.R. China
3Shanghai Institute of Applied Physcis, Chinese Academy of Sciences, Shanghai 201800, P.R. China
*Corresponding author: Email: gaoxw@njau.edu.cn
Shuai Wang1
1Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Education, Nanjing 210095, P.R. China
2Jinling Institute of Technology, Nanjing 211169, P.R. China
3Shanghai Institute of Applied Physcis, Chinese Academy of Sciences, Shanghai 201800, P.R. China
*Corresponding author: Email: gaoxw@njau.edu.cn
Xiaojuan Zhan2
1Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Education, Nanjing 210095, P.R. China
2Jinling Institute of Technology, Nanjing 211169, P.R. China
3Shanghai Institute of Applied Physcis, Chinese Academy of Sciences, Shanghai 201800, P.R. China
*Corresponding author: Email: gaoxw@njau.edu.cn
Xuewen Gao1
1Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Education, Nanjing 210095, P.R. China
2Jinling Institute of Technology, Nanjing 211169, P.R. China
3Shanghai Institute of Applied Physcis, Chinese Academy of Sciences, Shanghai 201800, P.R. China
*Corresponding author: Email: gaoxw@njau.edu.cn
The chitosan-HarpinXooc nanoparticles were prepared by the electrostatic interaction of chitosan (CS) with HarpinXooc and the ionic gelation of chitosan with tripolyphosphate (TPP). The particle sizes (197-653 nm) and zeta potential (29-15 mV) of the nanoparticles, which were changed with the preparing conditions such as the pH value (4.8-6.8), CS/TPP mass ratio (20:1-6:1) and HarpinXooc concentration (50-400 μg/mL), were determined respectively by dynamic light scattering and zeta potential analyzer. These preparing conditions were also examined systematically for their effects on the particles physicochemical properties such as encapsulation efficiency, loading capacity and release behavior at 26 ± 2 °C. The morphology and microstructure of the chitosan-tripolyphosphate nanoparticles were revealed by the transmission electron microscope analysis. This study demonstrated that systematic design and modulation of the chitosan-HarpinXooc nanoparticles could be readily achieved with the right control of processing conditions and would provide a sound basis to further exploration of HarpinXooc loaded nanoparticles.
Keywords
HarpinXoocChitosanNanoparticles.
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Zhan1, J., Wu1, H., Yang1, Y., Wang1, S., Zhan2, X., & Gao1, X. (2013). Preparation and Characterization of Chitosan-HarpinXooc Nanoparticles. Asian Journal of Chemistry, 25(18), 10368–10372. https://doi.org/10.14233/ajchem.2013.15507
