1State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110164, P.R. China
2University of Chinese Academy of Sciences, Beijing 100049, P.R. China
3Shandong Provincial Key Laboratory of Water and Soil Conservation & Environmental Protection, Linyi University, Linyi 276000, P.R. China
4Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, P.R. China
*Corresponding author: Fax: +86 24 83970300; Tel: +86 24 83970355; E-mail: ljchenchina@hotmail.com; ljchen@iae.ac.cn; gnzhangchina@gmail.com
Z.H. Chen1
1State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110164, P.R. China
2University of Chinese Academy of Sciences, Beijing 100049, P.R. China
3Shandong Provincial Key Laboratory of Water and Soil Conservation & Environmental Protection, Linyi University, Linyi 276000, P.R. China
4Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, P.R. China
*Corresponding author: Fax: +86 24 83970300; Tel: +86 24 83970355; E-mail: ljchenchina@hotmail.com; ljchen@iae.ac.cn; gnzhangchina@gmail.com
A.M. Zhang1
1State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110164, P.R. China
2University of Chinese Academy of Sciences, Beijing 100049, P.R. China
3Shandong Provincial Key Laboratory of Water and Soil Conservation & Environmental Protection, Linyi University, Linyi 276000, P.R. China
4Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, P.R. China
*Corresponding author: Fax: +86 24 83970300; Tel: +86 24 83970355; E-mail: ljchenchina@hotmail.com; ljchen@iae.ac.cn; gnzhangchina@gmail.com
L.J. Chen1
1State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110164, P.R. China
2University of Chinese Academy of Sciences, Beijing 100049, P.R. China
3Shandong Provincial Key Laboratory of Water and Soil Conservation & Environmental Protection, Linyi University, Linyi 276000, P.R. China
4Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, P.R. China
*Corresponding author: Fax: +86 24 83970300; Tel: +86 24 83970355; E-mail: ljchenchina@hotmail.com; ljchen@iae.ac.cn; gnzhangchina@gmail.com
Z.J. Wu4
1State Key Laboratory of Forest and Soil Ecology, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110164, P.R. China
2University of Chinese Academy of Sciences, Beijing 100049, P.R. China
3Shandong Provincial Key Laboratory of Water and Soil Conservation & Environmental Protection, Linyi University, Linyi 276000, P.R. China
4Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, P.R. China
*Corresponding author: Fax: +86 24 83970300; Tel: +86 24 83970355; E-mail: ljchenchina@hotmail.com; ljchen@iae.ac.cn; gnzhangchina@gmail.com
Soil properties and five hydrolytic enzyme activities under different simulated nitrogen deposition treatments were determined under semi-arid grassland in Northern China. Significant acidification was observed under N addition treatments. Highest values of soil total carbon, phosphorus and olsen-P concentration was observed in medium level of N application. Soil acid phosphomonoesterase, phosphodiesterase and N-acetyl-b-D-glucosaminidase activities increased with N amendment. The b-D-glucosidase and arylsulfatase activities were stimulated under medium level of N addition. The principal components analysis showed that N deposition might influence soil enzyme activities indirectly through soil pH, total carbon, total phosphorus and olsen-P concentration. Furthermore, the principal component analysis also showed similarity of N2.8 and N4.0 treatments, which indicated N saturation at 2.8 mol N m-2 yr-1 in the study area.
Zhang1, G., Chen1, Z., Zhang1, A., Chen1, L., & Wu4, Z. (2013). Effects of Nitrogen Deposition on Typical Hydrolytic Enzyme Activities by Fluorimetric Assay. Asian Journal of Chemistry, 25(18), 10335–10338. https://doi.org/10.14233/ajchem.2013.15384
