Antibacterial Property of Hydroxyapatite with Ag+ Dopant Produced by Hydrothermal Process

Abstract

The antimicrobial powders of hydroxyapatite with Ag⁺ dopant were one-step synthesized with Ca(NO₃)₂·4H₂O and AgNO₃, (NH₄)₂HPO₄ by hydrothermal method. It can be found that the concentration of Ag⁺ ions in hydroxyapatite powder has good linear relationship with the dosage of AgNO₃. The analyses of XRD patterns show that the Ag-hydroxyapatite powder has the same crystal structure and functional group with hydroxyapatite powder, which indicates that the Ag-hydroxyapatite powder exhibits the structural properties of pure hydroxyapatite powder. Through calculations of EDS and XRF, it is known that the Ca²⁺ ions in crystal structure of hydroxyapatite powder can be substituted partially by Ag⁺ ions in hydrothermal condition, which synthesize the final product Ag_xCa_10-x(PO₄)₆(OH)₂. The result of antibacterial experiment shows that the produced Ag-hydroxyapatite powder has good antibacterial property, the MIC £ 50 μg/mL (Escherichia coli and Staphylococcus aureus).