Issue

Vol. 25 No. 18 (2013)

Characterization of Protostane Triterpenoids in Dried Tuber of Alisma orientalis by Q-TOF Mass Spectrometry in Both Positive and Negative Modes

https://doi.org/10.14233/ajchem.2013.15286
Chengwu Song1
1Key Laboratory of Chinese Medicine Resource and Compound Prescription (Hubei University of Chinese Medicine), Ministry of Education, 1 Huang-jia-hu, Wuhan 430065, P.R. China 2Technology Center of China Tobacco Hubei Industry Corporation, Wuhan 430040, P.R. China 3Wuhan Huanghelou Flavors & Fragrances Co. Ltd., Wuhan 430040, P.R. China 4Wuhan Hospital of Traditional Chinese Medicine, 49 Lihuangpi Road, Jiangan District, Wuhan 430014, P.R. China 5Industrial Park Huaian District, Zhenhai Road, Jiangsu 223200, P.R. China *Corresponding author: Tel/Fax: +86 27 68890247; E-mail: yu20110425@hotmail.com †These authors contributed equally to this work.
Long Huang2
1Key Laboratory of Chinese Medicine Resource and Compound Prescription (Hubei University of Chinese Medicine), Ministry of Education, 1 Huang-jia-hu, Wuhan 430065, P.R. China 2Technology Center of China Tobacco Hubei Industry Corporation, Wuhan 430040, P.R. China 3Wuhan Huanghelou Flavors & Fragrances Co. Ltd., Wuhan 430040, P.R. China 4Wuhan Hospital of Traditional Chinese Medicine, 49 Lihuangpi Road, Jiangan District, Wuhan 430014, P.R. China 5Industrial Park Huaian District, Zhenhai Road, Jiangsu 223200, P.R. China *Corresponding author: Tel/Fax: +86 27 68890247; E-mail: yu20110425@hotmail.com †These authors contributed equally to this work.
Xiaofei Huang1
1Key Laboratory of Chinese Medicine Resource and Compound Prescription (Hubei University of Chinese Medicine), Ministry of Education, 1 Huang-jia-hu, Wuhan 430065, P.R. China 2Technology Center of China Tobacco Hubei Industry Corporation, Wuhan 430040, P.R. China 3Wuhan Huanghelou Flavors & Fragrances Co. Ltd., Wuhan 430040, P.R. China 4Wuhan Hospital of Traditional Chinese Medicine, 49 Lihuangpi Road, Jiangan District, Wuhan 430014, P.R. China 5Industrial Park Huaian District, Zhenhai Road, Jiangsu 223200, P.R. China *Corresponding author: Tel/Fax: +86 27 68890247; E-mail: yu20110425@hotmail.com †These authors contributed equally to this work.
Rongzeng Huang1
1Key Laboratory of Chinese Medicine Resource and Compound Prescription (Hubei University of Chinese Medicine), Ministry of Education, 1 Huang-jia-hu, Wuhan 430065, P.R. China 2Technology Center of China Tobacco Hubei Industry Corporation, Wuhan 430040, P.R. China 3Wuhan Huanghelou Flavors & Fragrances Co. Ltd., Wuhan 430040, P.R. China 4Wuhan Hospital of Traditional Chinese Medicine, 49 Lihuangpi Road, Jiangan District, Wuhan 430014, P.R. China 5Industrial Park Huaian District, Zhenhai Road, Jiangsu 223200, P.R. China *Corresponding author: Tel/Fax: +86 27 68890247; E-mail: yu20110425@hotmail.com †These authors contributed equally to this work.
Min Peng4
1Key Laboratory of Chinese Medicine Resource and Compound Prescription (Hubei University of Chinese Medicine), Ministry of Education, 1 Huang-jia-hu, Wuhan 430065, P.R. China 2Technology Center of China Tobacco Hubei Industry Corporation, Wuhan 430040, P.R. China 3Wuhan Huanghelou Flavors & Fragrances Co. Ltd., Wuhan 430040, P.R. China 4Wuhan Hospital of Traditional Chinese Medicine, 49 Lihuangpi Road, Jiangan District, Wuhan 430014, P.R. China 5Industrial Park Huaian District, Zhenhai Road, Jiangsu 223200, P.R. China *Corresponding author: Tel/Fax: +86 27 68890247; E-mail: yu20110425@hotmail.com †These authors contributed equally to this work.
Zhiyong Le5
1Key Laboratory of Chinese Medicine Resource and Compound Prescription (Hubei University of Chinese Medicine), Ministry of Education, 1 Huang-jia-hu, Wuhan 430065, P.R. China 2Technology Center of China Tobacco Hubei Industry Corporation, Wuhan 430040, P.R. China 3Wuhan Huanghelou Flavors & Fragrances Co. Ltd., Wuhan 430040, P.R. China 4Wuhan Hospital of Traditional Chinese Medicine, 49 Lihuangpi Road, Jiangan District, Wuhan 430014, P.R. China 5Industrial Park Huaian District, Zhenhai Road, Jiangsu 223200, P.R. China *Corresponding author: Tel/Fax: +86 27 68890247; E-mail: yu20110425@hotmail.com †These authors contributed equally to this work.
Ye Song1
1Key Laboratory of Chinese Medicine Resource and Compound Prescription (Hubei University of Chinese Medicine), Ministry of Education, 1 Huang-jia-hu, Wuhan 430065, P.R. China 2Technology Center of China Tobacco Hubei Industry Corporation, Wuhan 430040, P.R. China 3Wuhan Huanghelou Flavors & Fragrances Co. Ltd., Wuhan 430040, P.R. China 4Wuhan Hospital of Traditional Chinese Medicine, 49 Lihuangpi Road, Jiangan District, Wuhan 430014, P.R. China 5Industrial Park Huaian District, Zhenhai Road, Jiangsu 223200, P.R. China *Corresponding author: Tel/Fax: +86 27 68890247; E-mail: yu20110425@hotmail.com †These authors contributed equally to this work.
Shanggong Yu1
1Key Laboratory of Chinese Medicine Resource and Compound Prescription (Hubei University of Chinese Medicine), Ministry of Education, 1 Huang-jia-hu, Wuhan 430065, P.R. China 2Technology Center of China Tobacco Hubei Industry Corporation, Wuhan 430040, P.R. China 3Wuhan Huanghelou Flavors & Fragrances Co. Ltd., Wuhan 430040, P.R. China 4Wuhan Hospital of Traditional Chinese Medicine, 49 Lihuangpi Road, Jiangan District, Wuhan 430014, P.R. China 5Industrial Park Huaian District, Zhenhai Road, Jiangsu 223200, P.R. China *Corresponding author: Tel/Fax: +86 27 68890247; E-mail: yu20110425@hotmail.com †These authors contributed equally to this work.
Nianbai Fang1
1Key Laboratory of Chinese Medicine Resource and Compound Prescription (Hubei University of Chinese Medicine), Ministry of Education, 1 Huang-jia-hu, Wuhan 430065, P.R. China 2Technology Center of China Tobacco Hubei Industry Corporation, Wuhan 430040, P.R. China 3Wuhan Huanghelou Flavors & Fragrances Co. Ltd., Wuhan 430040, P.R. China 4Wuhan Hospital of Traditional Chinese Medicine, 49 Lihuangpi Road, Jiangan District, Wuhan 430014, P.R. China 5Industrial Park Huaian District, Zhenhai Road, Jiangsu 223200, P.R. China *Corresponding author: Tel/Fax: +86 27 68890247; E-mail: yu20110425@hotmail.com †These authors contributed equally to this work.

Corresponding Author(s) : Chengwu Song1

Asian Journal of Chemistry, Vol. 25 No. 18 (2013)

Abstract

Dried tuber of Alisma orientalis (Ze Xie) is a traditional Chinese medicine. It has been characterized by high concentrations of protostane triterpenoids, which are believed to play a crucial role for its clinical curative effect. This study describes a reliable and sensitive method for the analysis of protostane triterpenoids in Dried tuber of Alisma orientalis. The protostane triterpenoids were studied by HPLC/Q-TOF-mass spectrometry. A total of 28 protostane triterpenoids were simultaneously detected in Q-TOF-MS and 15 of them were newly found in Ze Xie. Especially, we focused on the CID MS/MS fragmentation patterns of 3 major components with different MS/MS collision energy at 15 or 35 eV and obtained extensive fragmentation information in structural elucidation. This is the first report regarding to the CID MS/MS fragmentation patterns of protostane triterpenoids in Ze Xie with both positive and negative modes. The results suggest that the method could be applied to analysis of protostane triterpenoids in Ze Xie and other herbs.

Keywords

Alisma orientale Ze Xie Protostane triterpenoids Q-TOF-MS.
Song1, C., Huang2, L., Huang1, X., Huang1, R., Peng4, M., Le5, Z., … Fang1, N. (2013). Characterization of Protostane Triterpenoids in Dried Tuber of Alisma orientalis by Q-TOF Mass Spectrometry in Both Positive and Negative Modes. Asian Journal of Chemistry, 25(18), 10296–10304. https://doi.org/10.14233/ajchem.2013.15286
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