1Comprehensive Utilization Key Laboratory of Chinese Herbal Medicine of Yueyang Dongting Lake Area, Yueyang Vocational & Technical College, Yueyang 414000, Hunan Province, P.R. China
2College of Material and Engineering, Central South University of Forestry and Technology, Changsha 410004, Hunan Province, P.R. China
*Corresponding author: Fax: +86 730 8677405; Tel: +86 13037302032; E-mail: sdlyxc@163.com
Delin Sun2
1Comprehensive Utilization Key Laboratory of Chinese Herbal Medicine of Yueyang Dongting Lake Area, Yueyang Vocational & Technical College, Yueyang 414000, Hunan Province, P.R. China
2College of Material and Engineering, Central South University of Forestry and Technology, Changsha 410004, Hunan Province, P.R. China
*Corresponding author: Fax: +86 730 8677405; Tel: +86 13037302032; E-mail: sdlyxc@163.com
Xijun Fu1
1Comprehensive Utilization Key Laboratory of Chinese Herbal Medicine of Yueyang Dongting Lake Area, Yueyang Vocational & Technical College, Yueyang 414000, Hunan Province, P.R. China
2College of Material and Engineering, Central South University of Forestry and Technology, Changsha 410004, Hunan Province, P.R. China
*Corresponding author: Fax: +86 730 8677405; Tel: +86 13037302032; E-mail: sdlyxc@163.com
Sirou Zhou1
1Comprehensive Utilization Key Laboratory of Chinese Herbal Medicine of Yueyang Dongting Lake Area, Yueyang Vocational & Technical College, Yueyang 414000, Hunan Province, P.R. China
2College of Material and Engineering, Central South University of Forestry and Technology, Changsha 410004, Hunan Province, P.R. China
*Corresponding author: Fax: +86 730 8677405; Tel: +86 13037302032; E-mail: sdlyxc@163.com
Tannin was extracted from wild persimmon leaves (Diospyros kaki L.f ) using the ultrasonic-assisted semi-bionic approach. Infrared spectrum analysis showed that tannins from wild persimmon leaves contain many proanthocyanidin-like substances with 3-O-gallate acyl structure. Free radical scavenging capacity assay showed that the ultrasonic-assisted semi-bionic approach can improve the scavenging activity of tannins on radicals, including OH•, DPPH• and ABTS•+. The half maximal inhibitory concentration values were reduced by 0.62, 0.116 and 1.19 mg mL-1, respectively, compared with the water reflux extraction method. Experimental results of orthogonal optimization showed that the tannin extraction quantity can reach 226.8 mg g-1, which was much higher than the sample obtained without ultrasonic assistance under the following experimental conditions: pH values of 1.8, 7.5 and 8.4, respectively; 50 % solvent mass fraction; 35 min extraction time; 50 ºC extraction temperature; 300 W ultrasonic power and 30 KHz ultrasonic frequency. These results suggested that this method could increase the activity and extraction yield of tannins.
Yu1, X., Sun2, D., Fu1, X., & Zhou1, S. (2013). Ultrasonic-Assisted Semi-Bionic Extraction of Tannins from Wild Persimmon Leaves. Asian Journal of Chemistry, 25(18), 10191–10194. https://doi.org/10.14233/ajchem.2013.15223
