1State Key Laboratory of Hollow Fiber Membrane Materials and Processes, School of Environment and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300387, P.R. China
2Department of Physiological Sciences, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, Oklahoma 74078, United States of America
*Corresponding author: Fax: +86 22 83955451; Tel: +86 22 83955167; E-mail: toby509@163.com; zhangjimei6d311@163.com
Jimei Zhang1
1State Key Laboratory of Hollow Fiber Membrane Materials and Processes, School of Environment and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300387, P.R. China
2Department of Physiological Sciences, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, Oklahoma 74078, United States of America
*Corresponding author: Fax: +86 22 83955451; Tel: +86 22 83955167; E-mail: toby509@163.com; zhangjimei6d311@163.com
Guangping Chen1
1State Key Laboratory of Hollow Fiber Membrane Materials and Processes, School of Environment and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300387, P.R. China
2Department of Physiological Sciences, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, Oklahoma 74078, United States of America
*Corresponding author: Fax: +86 22 83955451; Tel: +86 22 83955167; E-mail: toby509@163.com; zhangjimei6d311@163.com
Ying Li1
1State Key Laboratory of Hollow Fiber Membrane Materials and Processes, School of Environment and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300387, P.R. China
2Department of Physiological Sciences, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, Oklahoma 74078, United States of America
*Corresponding author: Fax: +86 22 83955451; Tel: +86 22 83955167; E-mail: toby509@163.com; zhangjimei6d311@163.com
Shibo Qi2
1State Key Laboratory of Hollow Fiber Membrane Materials and Processes, School of Environment and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300387, P.R. China
2Department of Physiological Sciences, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, Oklahoma 74078, United States of America
*Corresponding author: Fax: +86 22 83955451; Tel: +86 22 83955167; E-mail: toby509@163.com; zhangjimei6d311@163.com
Li Li2
1State Key Laboratory of Hollow Fiber Membrane Materials and Processes, School of Environment and Chemical Engineering, Tianjin Polytechnic University, Tianjin 300387, P.R. China
2Department of Physiological Sciences, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, Oklahoma 74078, United States of America
*Corresponding author: Fax: +86 22 83955451; Tel: +86 22 83955167; E-mail: toby509@163.com; zhangjimei6d311@163.com
A biosensor by a covalent self-assembly process was constructed to detect the DNA sequences of genetically modified corn based on electrochemical detection. This novel detection system consisted of the sulfhydryl-functionalized multi-walled carbon nanotubes, gold nanoparticles, DNA sequences and gold electrodes. The self-assembly processes and DNA detection were analyzed by cyclic voltammetry and different pulse voltammetry. The results showed that the DNA biosensor offered a higher selectivity and sensitivity with the detection limit of 4.03 × 10-10 mol/L. Moreover, the biosensor has great potential to provide a general method for detecting DNA sequences of many other genetically modified organisms.
Keywords
DNA biosensorMulti-walled carbon nanotubesGold nanoparticlesGenetically modified organism.
Full Article
Generated from XML file
Tian1, L., Zhang1, J., Chen1, G., Li1, Y., Qi2, S., & Li2, L. (2013). Covalent Self-Assembly of Multi-Walled Carbon Nanotubes and Gold Nanoparticles for Detecting DNA Sequences of Genetically Modified Corn. Asian Journal of Chemistry, 25(18), 10535–10540. https://doi.org/10.14233/ajchem.2013.15846
