1Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, P.R. China
2Graduate University of Chinese Academy of Sciences, Beijing 100049, P.R. China
3Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, P.R. China
4Huzhou Plateau Biological Resource Centre of Innovation, Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Huzhou 310001, P.R. China
*Corresponding author: E-mail: designdl@163.com
Tong-Yong Zhou
Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, P.R. China ; Graduate University of Chinese Academy of Sciences, Beijing 100049, P.R. China ; Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, P.R. China
Li Pi
Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, P.R. China ; Graduate University of Chinese Academy of Sciences, Beijing 100049, P.R. China ; Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, P.R. China
Xiao-Hui Zhao
Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, P.R. China ; Graduate University of Chinese Academy of Sciences, Beijing 100049, P.R. China ; Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, P.R. China
Tao Han
Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, P.R. China ; Graduate University of Chinese Academy of Sciences, Beijing 100049, P.R. China ; Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, P.R. China
Yi-Kang Li
Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, P.R. China ; Graduate University of Chinese Academy of Sciences, Beijing 100049, P.R. China ; Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, P.R. China
Fa Han
Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, P.R. China ; Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, P.R. China
Corresponding Author(s) : Fa Han
designdl@163.com
Asian Journal of Chemistry,
Vol. 25 No. 14 (2013): Vol 25 Issue 14
The response surface methodology was employed to optimize the integrated extraction parameters of cordycepic acid and cordycepin from cultured Cordyceps militaris (L.) link based on a single-factor experiment. The Box-Behnken design with three independent variables i.e., microwave power (W), water/material ratio (mL/g) and extraction time (min) was used. The experimental data obtained were fitted to a second-order polynomial equation using multiple regression analysis. The three-dimensional response surface plot and contour plot derived from the mathematical models were applied to determine the optimal conditions. The optimum extraction condition was obtained as follows: microwave-assisted extraction, microwave power of 649.33 W, extraction time of 5.74 min, water/material ratio of 38. 99 mL/g and extraction number of three. The yield of cordycepic acid and cordycepin were 2.47 and 0.79 %, respectively. Under these conditions, the experimental values of 3.12 and 0.75 % well agreed with those predicted by the model.
Deng1, L., Zhou, T.-Y., Pi, L., Zhao, X.-H., Han, T., Li, Y.-K., & Han, F. (2013). Optimization of Microwave-Assisted Extraction of Cordycepic Acid and Cordycepin from Cultured Cordyceps militaris by Response Surface Methodology. Asian Journal of Chemistry, 25(14), 8065–8071. https://doi.org/10.14233/ajchem.2013.15056
