Institute for Drug and Instrument Control of Health Department GLD of PLA, No. 17 Fengtai West Road, Beijing 100071, P.R. China ; These authors have equal contribution to this work. Both persons are the first authors.
Ming Zhao
School of Pharmacy, Shenyang Pharmaceutical University, No. 103 Wenhua Road, Shenyang 110016, P.R. China ; Clinical Pharmaceutics Room, The People's Hospital of Wenshan Prefecture, No. 228 Kaihua Middle Road, Wenshan 663000, P.R. China ; These authors have equal contribution to this work. Both persons are the first authors.
Xiaoying Fu
Institute for Drug and Instrument Control of Health Department GLD of PLA, No. 17 Fengtai West Road, Beijing 100071, P.R. China
Jiong Zhang
Institute for Drug and Instrument Control of Health Department GLD of PLA, No. 17 Fengtai West Road, Beijing 100071, P.R. China
Yang Ji
Institute for Drug and Instrument Control of Health Department GLD of PLA, No. 17 Fengtai West Road, Beijing 100071, P.R. China
Xiongping Jiang
Institute for Drug and Instrument Control of Health Department GLD of PLA, No. 17 Fengtai West Road, Beijing 100071, P.R. China
Xiaofei Fu
Institute for Drug and Instrument Control of Health Department GLD of PLA, No. 17 Fengtai West Road, Beijing 100071, P.R. China
Chunjie Zhao
School of Pharmacy, Shenyang Pharmaceutical University, No. 103 Wenhua Road, Shenyang 110016, P.R. China
Corresponding Author(s) : Chunjie Zhao
chenlei00000@126.com
Asian Journal of Chemistry,
Vol. 25 No. 14 (2013): Vol 25 Issue 14
In the present study a method based on liquid chromatography with diode array detection (HPLC-DAD) coupled to an electrospray ionization (ESI) interface for identify the chemical structures of related substances in citicoline sodium injection was established. There are seven impurities in a typical chromatogram of citicoline sodium injection (5'-CMP has known). The UV spectrum and molecular weight of related substances were obtained and the chemical structures were presumed according to production process. Compared with reference substances, two impurities were confirmed as cytosine and cytidine. As for the largest related substance (except 5'-CMP), it was collected by liquid chromatography and treated by solid-phase extraction for LC-MS system. Compared with self made reference substance, it was confirmed as uridine diphosphate choline. The three main related substances were identified in citicoline sodium injection for the first time.
Gao, J., Zhao, M., Fu, X., Zhang, J., Ji, Y., Jiang, X., … Zhao, C. (2013). Identification of Related Substances in Citicoline Sodium Injection by HPLC-DAD and HPLC-MS/MS. Asian Journal of Chemistry, 25(14), 7697–7704. https://doi.org/10.14233/ajchem.2013.14572
