Evaluation of Phytochemical and Anti-oxidant Activity in Different Mulberry Varieties

Abstract

The aims of present study were to screen phytochemical constituents and evaluate in vitro anti-oxidant activity of leaves of different varieties of mulberry plant [Family Moraceae]. The methanolic extract of leaves of different mulberry varieties namely S-13, S-54, BR-2, S-36 and S-1 which belongs to Morus alba and Morus indica were subjected for the phytochemical screening. The results obtained from the HPLC analysis, revealed that the methanolic extracts of mulberry leaves samples of S-36 and S-1 varieties contain more amount of phenolics and flavonoids. The in vitro FRAP assay clearly indicate that S-1 mulberry leaf extract have maximum significant FRAP concentration at 800 μL/mL and 1000 μL/mL (0.408 ± 0.001 mmol FeSO₄/100 g dried sample and 0.410 ± 0.001 mmol FeSO₄/100 g dried sample, respectively) a significant source of natural anti-oxidant, which might be helpful in preventing the progress of various oxidative stresses. However, the components responsible for the antioxidative activity are morin, querectin, resveratrol, scopoletin, 7-hydroxycoumarin. Furthermore, these mulberry varieties may be utilized in epidemiological studies.