Chiral Separation of Lamivudine by Capillary Zone Electrophoresis

Abstract

In this work, the application of capillary electrophoresis in chiral technology was studied for lamivudine. A new capillary zone electrophoresis method was established to chiral separation of lamivudine. It was separated with an untreated fused silica capillary (75 μm I.D., effective length is 55 cm). 40 mmol/L sodium dihydrogen phosphate and 40 mg/mL HP-b-cyclodextrin (pH = 3.1) was running buffer solution at the detection wavelength of 270 nm. Separation voltage was 15 kv and the injection time of sample was 15 s. The separation of both enantiomers of lamivudine could be achieved baseline resolution. The calibration curve for lamivudine optical isomers exhibited an excellent linear relationship in concentration range of 0.0625 to 1 mg/mL. The precision was less than 2 %. The average recovery was 99.71 % and 100.12 %. In addition, the detection limit was 1.25 μg/mL for lamivudine and its enantiomer was 2.5 μg/mL. The method is simple, rapid and accurate. It can be used for quality control of the enantiomers of lamivudine.