Qualitative Determination of Known Thromboxane Receptors in Human Plasma by LC-MS (Quadrupole Time of Flight)

Abstract

A simple, rapid and sensitive reverse-phase LC-MS method has been developed for the simultaneous quantification of Dazmegrel, GR 32191, SQ-29548 and U-46619 in human plasma. Analytes were extracted from human plasma by liquid-liquid extraction technique using acetonitrile. Separation was achieved on a reverse phase Shodex C18, 3 μm, 4.6 × 100 mm column at ambient temperature under isocratic conditions at a flow rate of 1 mL/min, run time of 10 min with acetonitrile and 0.1 % formic acid in water (70:30 v/v). Detection was performed using dual electrospray ionization (ESI) by using a fast polarity switch mode for fragment ions and adduct as for Dazmegrel, GR32191, SQ-29548 and U-46619 in positive and negative modes. The results indicate that this proposed method is convenient and reliable for clinical studies application.