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Vol. 25 No. 13 (2013): Vol 25 Issue 13

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DNA Binding and Oxidative Cleavage Studies of Supramolecular Copper(II) Complex Based on b-Cyclodextrin

https://doi.org/10.14233/ajchem.2013.14707
Si-Ping Tang
Key Laboratory of Functional Organometallic Materials, Department of Chemistry and Material Science, Hengyang Normal University, Hengyang 421008, Hunan Province, P.R. China
Yong-Lan Feng
Key Laboratory of Functional Organometallic Materials, Department of Chemistry and Material Science, Hengyang Normal University, Hengyang 421008, Hunan Province, P.R. China
Zhi-Feng Xu
Key Laboratory of Functional Organometallic Materials, Department of Chemistry and Material Science, Hengyang Normal University, Hengyang 421008, Hunan Province, P.R. China
De-Ping Wang
Key Laboratory of Functional Organometallic Materials, Department of Chemistry and Material Science, Hengyang Normal University, Hengyang 421008, Hunan Province, P.R. China

Corresponding Author(s) : Si-Ping Tang

tsp00@163.com

Asian Journal of Chemistry, Vol. 25 No. 13 (2013): Vol 25 Issue 13

Abstract

A copper(II) complex (CuL, 1) with aminothioether ligand and its new inclusion complex with b-cyclodextrin (CuL/b-CD, 2) have been prepared successfully. The binding modes of both complexes with calf thymus DNA were investigated by UV-visible and circular dichroism spectroscopies. Results show that both complexes mainly adopt electrostatic attraction binding mode with DNA and binding constants are (2.02 ± 0.02) × 103 and (1.79 ± 0.05) × 103 M-1, respectively. Both complexes are able to cleave pBR322 plasmid DNA efficiently in the presence of ascorbic acid and the activity of 2 is higher than that of 1. The DNA cleavages by 1 and 2 were inhibited strongly in the presence of DMSO and tert-butyl alcohol, which suggests that hydroxyl radicals are reactive oxygen species for the cleavage.

Keywords

Copper(II) complex Inclusion complex DNA binding Nuclease activity
Tang, S.-P., Feng, Y.-L., Xu, Z.-F., & Wang, D.-P. (2013). DNA Binding and Oxidative Cleavage Studies of Supramolecular Copper(II) Complex Based on b-Cyclodextrin. Asian Journal of Chemistry, 25(13), 7397–7400. https://doi.org/10.14233/ajchem.2013.14707
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