Simultaneous Determination of Five Active Ingredients in Yiqing Tablet by HPLC

Abstract

To establish an HPLC method for the simultaneous determination of 5 active ingredients in Yiqing tablet. HPLC conditions included Agilent ZorbaxSB-C18 column (4.6 mm × 250 mm, 5 μm) and the mobile phase was a mixture of acetonitrile and 0.1 % formic acid for gradient elution.The flow rate was 1 mL/min and the column temperature was 35 ºC. Five active ingredients had good linear relationships: berberinein the range of 0.0024-0.1192 μg (R² = 0.9995), aloe-emodin in the range of 0.00103-0.0515 μg (R² = 0.9998), emodin in the range of 0.0052-0.0260 μg (R² = 0.9999), chrysophanol in the range of 0.0011-0.0560 μg (R² = 0.9999) and physcion in the range of 0.0011-0.0530 μg (R² = 0.9996). Their average recoveries were 101.4 % (RSD = 2.3 %), 100.6 % (RSD = 2.0 %), 100.3 % (RSD = 2.8 %), 102.0 % (RSD = 1.6 %) and 101.8 % (RSD = 1.8 %), respectively. This method was steady with high precision and good repeatability and could be used for the determination of the five active ingredients and quality control of Yiqing tablet.