Copyright (c) 2013 AJC
This work is licensed under a Creative Commons Attribution 4.0 International License.
Identification and Characterization of Constituents in Si-Wu-Tang by Liquid Chromatography Connected with Time of Flight Mass Spectrometry and Ion Trap Mass Spectrometry
Corresponding Author(s) : Changxiao Liu
Asian Journal of Chemistry,
Vol. 25 No. 11 (2013): Vol 25 Issue 11
Abstract
A combination of high performance liquid chromatography time of flight mass spectrometry (HPLC-TOF/MS) and high performance liquid chromatography coupled with diode array detection and electrospray ionization tandem mass spectrometry (HPLC-DAD-ESI-MS/MS) as a powerful method to profile and identify non-target components in traditional Chinese medicine (TCM) was developed. Si-Wu-Tang (SWT), a popular traditional Chinese medicine, was studied using the combinative method as for an application. Based on the mass spectra, UV spectra and retention time, 21 compounds were identified or tentatively characterized. This study represents the first detailed investigation of the components of Si-Wu-Tang with HPLC-TOF/MS technique. The identification and structural elucidation of the chemical constituents provided essential data for further quality control and pharmacological mechanism research of Si-Wu-Tang. With the introduction of the combinative HPLC-MS technique analytical system to traditional Chinese medicine, we expected that approach would be useful for the screening and characterization of non-target compounds in other famous traditional Chinese medicine.
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Y. Dai, P.PH. But, Y.P. Chan, H. Matsuda and M. Kubo, Biol. Pharm. Bull., 25, 1175 (2002).
Q.D. Liang, Y. Gao, H.L. Tan, P. Gou, Y.F. Li, Z. Zhou, W. Tan, Z.C. Ma, B.P. Ma and S.Q. Wang, Biol. Pharm. Bull., 29, 1378 (2006).
J.F. Cheng, Z.Y. Lu, Y.C. Su, L.C. Chang and R.Y. Wang, J. Clin. Nurs., 17, 2588 (2008).
H.J. Zhang, P. Shen and Y.Y. Cheng, J. Pharm. Biomed. Anal., 34, 705 (2004).
Y.P. Tang, M. Zhu, S. Yu, Y.Q. Hua, J.A. Duan, S.L. Su, X. Zhang, Y. Lu and A.W. Ding, Molecules, 15, 341 (2010).
H.P. Zeng, T.T. Wang, W. Chen, C.Y. Wang, D.F. Chen and J.G. Shen, Bioorg. Med. Chem., 16, 5109 (2008).
J.L. Zhou, L.W. Qi and P. Li, J. Chromatogr. A, 1216, 7582 (2009).
M. Yang, J.H. Sun, Z.Q. Lu, G.T. Chen, S.H. Guan, X. Liu, B.H. Jiang, M. Ye and D.A. Guo, J. Chromatogr. A, 1216, 2045 (2009).
Y.Y. Zhang, Q. Wang, L.W. Qi, X.Y. Qin and M.J. Qin, J. Pharm. Biomed. Anal., 56, 304 (2011).
Q. Tan, W.H. Wang, P. Song, L.M. Chen, J.J. Zhu, Z.M. Wang and J.C. Cui, China J. Chin. Mater. Med., 36, 687 (2011).
L. Yang, S.J. Xu, R.T. Tian, P.S. Xie and Z.T. Wang, Acta Pharm. Sin., 42, 71 (2007).
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T. Yi, K.S. Leung, G.H. Lu, K. Chan and H. Zhang, Chem. Pharm. Bull., 54, 255 (2006).