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Vol. 25 No. 11 (2013): Vol 25 Issue 11

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Phytochemical Analysis of Saponifiable Matter of Petroleum Ether Extract of Leaves of Butea monosperma

https://doi.org/10.14233/ajchem.2013.14236
Ambarsing P. Rajput
Department of Chemistry, Z.B. Patil College, Dhule-424 002, India
Bhagavan S. Patil
Department of Chemistry, G.M.C. Polytechnic, Shahada-425 409, India

Corresponding Author(s) : Ambarsing P. Rajput

aprajput@rediffmail.com

Asian Journal of Chemistry, Vol. 25 No. 11 (2013): Vol 25 Issue 11

Abstract

The present study designed for phytochemical analysis, antibacterial, antifungal activity of saponifiable matter of petroleum ether extract of leaves of Butea monosperma belongs to family Fabaceae. The extraction preformed by soxhlet extractor and identification was performed by phytochemical test. The petroleum ether extract of leaves of Butea monosperma was saponified using 1 M alcoholic KOH, to separate saponifiable (fatty material) and unsaponofibale matter. A comprehensive phytochemical investigation of the saponifiable matter of petroleum ether extract of leaves of Butea monosperma resulted in the isolation and identification of four known compounds Palmitic acid, tetradecanoic acid, oleic acid and a-linolenic acid. To our best of knowledge, these four compounds were isolated from leaves of plant for the first time. Their structures were identified by physical, chemical, elemental analysis and spectroscopic methods including UV, FTIR, MS, 1H NMR, 13C NMR. The study was further continued for screening of antibacterial activity against E. coli, P. aeruginosa, B. subtilis, S. aureus and antifungal activity screened against A. niger and C. albicans using agar diffusion assay.

Keywords

Butea monosperma Saponifiable matter Antibacterial activity Antifungal activity
P. Rajput, A., & S. Patil, B. (2013). Phytochemical Analysis of Saponifiable Matter of Petroleum Ether Extract of Leaves of Butea monosperma. Asian Journal of Chemistry, 25(11), 6016–6020. https://doi.org/10.14233/ajchem.2013.14236
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