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Vol. 25 No. 11 (2013): Vol 25 Issue 11

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Extractive Bioconversion of L-Phenylalanine to 2-Phenylethanol Using Polypropylene Glycol 1500

https://doi.org/10.14233/ajchem.2013.14201
Dong-Liang Hua
Key Laboratory for Biomass Gasification Technology of Shandong Province, Energy Research Institute of Shandong Academy of Sciences, Jinan 250014, P.R. China
Xiao-Hui Liang
Key Laboratory for Biomass Gasification Technology of Shandong Province, Energy Research Institute of Shandong Academy of Sciences, Jinan 250014, P.R. China
Cheng-Chuan Che
School of Life Sciences, Qufu Normal University, Qufu 273165, P.R. China
Xiao-Dong Zhang
Key Laboratory for Biomass Gasification Technology of Shandong Province, Energy Research Institute of Shandong Academy of Sciences, Jinan 250014, P.R. China
Jie Zhang
Key Laboratory for Biomass Gasification Technology of Shandong Province, Energy Research Institute of Shandong Academy of Sciences, Jinan 250014, P.R. China
Yan Li
Key Laboratory for Biomass Gasification Technology of Shandong Province, Energy Research Institute of Shandong Academy of Sciences, Jinan 250014, P.R. China
Ping Xu
School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, P.R. China

Corresponding Author(s) : Xiao-Dong Zhang

hua016@foxmail.com

Asian Journal of Chemistry, Vol. 25 No. 11 (2013): Vol 25 Issue 11

Abstract

Product cytotoxicity was a bottleneck in biotechnological production of 2-phenylethanol from L-phenylalanine. In this study, polypropylene glycol 1500 (PPG 1500) was firstly used as an extractant in the extractive bioconversion of L-phenylalanine by strain Saccharomyces cerevisiae P-3. About 4.8 g L-1 2-phenylethanol was obtained from 7 g L-1 L-phenylalanine in flask experiment within 36 h, with a high molar yield of 92.7 %. Compared to conventional bioconversion without addition of extractant, the yield was improved by 26.3 %. In a fed-batch extractive bioconversion, the highest concentration of 2-phenylethanol in the organic phase exceeded 22 g L-1 and the total concentration in both aqueous and organic phases reached 7.5 g L-1. The results showed that the process with polypropylene glycol 1500 as an extractant might have the potential in bioproduction of 2-phenylethanol.

Keywords

2-Phenylethanol L-phenylalanine Product cytotoxicity Polypropylene glycol 1500 Extractive bioconversion
Hua, D.-L., Liang, X.-H., Che, C.-C., Zhang, X.-D., Zhang, J., Li, Y., & Xu, P. (2013). Extractive Bioconversion of L-Phenylalanine to 2-Phenylethanol Using Polypropylene Glycol 1500. Asian Journal of Chemistry, 25(11), 5951–5954. https://doi.org/10.14233/ajchem.2013.14201
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