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Optimization of the Conditions for the Analysis of Curcumin and a Related Compound in Curcuma longa with Mobile-Phase Composition and Column Temperature via RP-HPLC
Corresponding Author(s) : J.Y. Ma
Asian Journal of Chemistry,
Vol. 25 No. 11 (2013): Vol 25 Issue 11
Abstract
To efficiently extract the curcuminoids from turmeric, the extracted samples were simultaneously separated and analyzed via commercial C18 reverse-phase high-performance liquid chromatography using combined water and acetonitrile as the mobile phase in the isocratic elution mode. The experiment results showed that the optimum mobile-phase composition of the water/acetonitrile was 60/40 vol % using the Knauer RP-column. The effect of the solvent composition (methanol in water) on the extraction yield, flow rate (1.0 mL/min), injection volume (10 μL) and column oven temperature (40 ºC) at a 425 nm wavelength was studied. It was evident that the amount of the curcuminoids that were extracted (extraction time: 4 h) with 100 % MeOH was higher than any aqueous MeOH composition. The correlation coefficients were greater than curcumin (1) 0.9985, demethoxycurcumin (2) 0.9982 and bisdemethoxycurcumin (3) 0.9990 in the regression analyses. The LC-MS data provided molecular weight information for the components in the curcumin standard (purity: 80 %). Thus, the optimum operating conditions were experimentally determined.
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X.C. Zhao, L. Zhang, H.X. Yu, Z. Sun, X.F. Lin, C. Tan and R.R. Lu, Food Chem., 129, 387 (2011).
J.K. Kim, C. Jo, H.J. Hwang, H.J. Park, Y.J. Kim, M.W. Byun, Radiat. Phys. Chem., 75, 449 (2006).
S.W. Oh, J.Y. Cha, J.E. Jung, B.C. Chang, H.J. Kwon, B.R. Lee and D.Y. Kim, J. Ethnopharmacol., 136, 414 (2011).
R.K. Singh, D. Rai, D. Yadav, A. Bhargava, J. Balzarini and E.D. Clercq, Eur. J. Med. Chem., 45, 1078 (2010).
S. Gilda, M. Kanitkar, R. Bhonde and A. Paradkar, LWT-Food Sci. Technol., 43, 59 (2010).
H. Jiang, B.N. Timmermann and D.R. Gang, J. Chromatogr. A, 1111, 21 (2006).
S. Pandit, H.J. Kim, J.E. Kim and J.G. Jeon, Food Chem., 126, 1565 (2011).
M. Cousins, J. Adelberg, F. Chen and J. Rieck, Ind. Crops Prod., 25, 129 (2007).
P. Anand, S.G. Thomas, A.B. Kunnumakkara, C. Sundaram, K.B. Harikumar, B. Sung, S.T. Tharakan, K. Misra, I.K. Priyadarsini, K.N. Rajasekharan and B.B. Aggarwal, Biochem. Pharm., 76, 1590 (2008).
V. Jain, V. Prasad, R. Pal and S. Singh, J. Pharm. Biomed. Anal., 44, 1079 (2007).
J.H. Lee and M.G. Choung, Food Chem., 124, 1217 (2011).